Wheat CBL-interacting protein kinase 23 positively regulates drought stress and ABA responses

被引：99

作者：

Cui, Xiao-Yu ^{[1
]}

Du, Yong-Tao ^{[1
]}

Fu, Jin-dong ^{[1
]}

Yu, Tai-Fei ^{[1
]}

Wang, Chang-Tao ^{[2
]}

Chen, Ming ^{[1
]}

Chen, Jun ^{[1
]}

Ma, You-Zhi ^{[1
]}

Xu, Zhao-Shi ^{[1
]}

机构：

[1] CAAS, Minist Agr,Inst Crop Sci, Key Lab Biol & Genet Improvement Triticeae Crops, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[2] Beijing Technol & Business Univ, Beijing Key Lab Plant Resource Res & Dev, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China

来源：

BMC PLANT BIOLOGY | 2018年 / 18卷

关键词：

ABA; CIPK; CBL; Drought tolerance; Induced mechanism; Wheat; ABIOTIC STRESS; ABSCISIC-ACID; ARABIDOPSIS-THALIANA; CALCIUM SENSOR; SALT TOLERANCE; TRANSCRIPTIONAL REGULATION; SIGNALING NETWORK; GENES; SOS2; RICE;

D O I：

10.1186/s12870-018-1306-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Background: The calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) signaling pathway responds to various abiotic stresses in plants. Results: Wheat CIPK23, isolated from wheat drought transcriptome data set, was induced by multiple abiotic stresses, including drought, salt, and abscisic acid (ABA). Compared with wild-type plants, TaCIPK23-overexpression wheat and Arabidopsis showed an higher survival rate under drought conditions with enhanced germination rate, developed root system, increased accumulation of osmolytes, and reduced water loss rate. Over-expression of TaCIPK23 rendered transgenic plants ABA sensitivity, as evidenced by delayed seed germination and the induction of stomatal closure. Consistent with the ABA-sensitive phenotype, the expression level of drought-and ABA-responsive genes were increased under drought conditions in the transgenic plants. In addition, using yeast two-hybrid system, pull-down and bimolecular fluorescence complementation (BiFc) assays, TaCIPK23 was found to interact with TaCBL1 on the plasma membrane. Conclusions: These results suggest that TaCIPK23 plays important roles in ABA and drought stress responses, and mediates crosstalk between the ABA signaling pathway and drought stress responses in wheat.

引用

页数：13

共 63 条

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