Micro-RNA detection based on fluorescence resonance energy transfer of DNA-carbon quantum dots probes

被引：52
作者：
Khakbaz, Faeze [1 ]
Mahani, Mohamad [1 ]
机构：
[1] Grad Univ Adv Technol, Fac Sci & Modern Technol, Dept Chem & Nanochem, Kerman, Iran
来源：
ANALYTICAL BIOCHEMISTRY | 2017年 / 523卷
关键词：
Carbon quantum dots; Mir; 9-1; FRET; Single stranded DNA; FAM; Quantum yield; REAL-TIME PCR; BREAST-CANCER; SENSITIVE DETECTION; EXPRESSION; NANOPARTICLES; AMPLIFICATION; BIOMARKERS; NANODOTS; SAMPLES; CELLS;
D O I：
10.1016/j.ab.2017.01.025
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Carbon quantum dots have been proposed as an effective platform for miRNA detection. Carbon dots were synthesized by citric acid. The synthesized dots were characterized by dynamic light scattering, UV-Vis spectrophotometry, spectrofluorimetry, transmission electron microscopy and FT-IR spectrophotometry. The fluorescence quantum yield of the synthesized dots was determined using quinine sulfate as the standard. The FAM-labeled single stranded DNA, as sensing element, was adsorbed on dots by pi-pi interaction. The quenching of the dots fluorescence due to fluorescence resonance energy transfer (FRET) was used for mir 9-1 detection. In the presence of the complementary miRNA, the FRET did not take place and the fluorescence was recovered. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：32 / 38
页数：7
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