Modulating Short Wavelength Fluorescence with Long Wavelength Light

被引：19

作者：

Copley, Graeme ^{[1
]}

Gillmore, Jason G. ^{[1
,2
]}

Crisman, Jeffrey ^{[1
]}

Kodis, Gerdenis ^{[1
]}

Gray, Christopher L. ^{[1
]}

Cherry, Brian R. ^{[3
]}

Sherman, Benjamin D. ^{[1
]}

Liddell, Paul A. ^{[1
]}

Paquette, Michelle M. ^{[4
]}

Kelbauskas, Laimonas ^{[5
]}

Frank, Natia L. ^{[4
]}

Moore, Ana L. ^{[1
]}

Moore, Thomas A. ^{[1
]}

Gust, Devens ^{[1
]}

机构：

[1] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

[2] Hope Coll, Dept Chem, Holland, MI 49423 USA

[3] Arizona State Univ, Magnet Resonance Res Ctr, Tempe, AZ 85287 USA

[4] Univ Victoria, Dept Chem, Victoria, BC V8W 3V6, Canada

[5] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 34期

基金：

美国国家科学基金会;

关键词：

PHOTOINDUCED ELECTRON-TRANSFER;

D O I：

10.1021/ja504879p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two molecules in which the intensity of shorter-wavelength fluorescence from a strong fluorophore is modulated by longer-wavelength irradiation of an attached merocyanine-spirooxazine reverse photochromic moiety have been synthesized and studied. This unusual fluorescence behavior is the result of quenching of fluorophore fluorescence by the thermally stable, open, zwitterionic form of the spirooxazine, whereas the photogenerated closed, spirocyclic form has no effect on the fluorophore excited state. The population ratio of the closed and open forms of the spirooxazine is controlled by the intensity of the longer-wavelength modulated light. Both square wave and sine wave modulation were investigated. Because the merocyanine-spirooxazine is an unusual reverse photochrome with a thermally stable long-wavelength absorbing form and a short-wavelength absorbing photogenerated isomer with a very short lifetime, this phenomenon does not require irradiation of the molecules with potentially damaging ultraviolet light, and rapid modulation of fluorescence is possible. Molecules demonstrating these properties may be useful in fluorescent probes, as their use can discriminate between probe fluorescence and various types of adventitious "autofluorescence" from other molecules in the system being studied.

引用

页码：11994 / 12003

页数：10