Fraction transfer process in on-line comprehensive two-dimensional liquid-phase separations

被引:38
作者
Cesla, Petr [1 ]
Krenkova, Jana [2 ]
机构
[1] Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Studentska 573, CZ-53210 Pardubice, Czech Republic
[2] CAS, Inst Analyt Chem, Vvi, Brno, Czech Republic
关键词
capillary electrophoresis; comprehensive liquid chromatography; fraction transfer; liquid chromatography; two-dimensional separations; FLIGHT MASS-SPECTROMETRY; CAPILLARY-ZONE-ELECTROPHORESIS; SUPERCRITICAL-FLUID CHROMATOGRAPHY; HYDROPHILIC INTERACTION; PEAK-CAPACITY; ZWITTERIONIC POLYMETHACRYLATE; EVAPORATION INTERFACE; MONOLITHIC COLUMNS; PHENOLIC-COMPOUNDS; PROTEOME ANALYSIS;
D O I
10.1002/jssc.201600921
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Two-dimensional liquid-phase separations have gained increasing attention for their ability to separate complex sample mixtures. Among the experimental setups used, an on-line approach is preferred to reduce the probability of sample contamination, for easier automation and high-sample throughput. The interfacing of the separation techniques in the on-line mode brings additional demands on proper optimization of the two-dimensional system. In this review, the possibilities of the on-line coupling of liquid chromatography and liquid chromatography with capillary electrophoresis in two-dimensional systems are discussed. Special attention is paid to the fraction transfer process, which includes an overview of interfaces and experimental setups applied, the compatibility issues of separation systems, and instrumental parameters. The benefits and drawbacks of using electromigration separations in combination with liquid chromatography are presented as well.
引用
收藏
页码:109 / 123
页数:15
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