Overexpression of Snail in retinal pigment epithelial triggered epithelial-mesenchymal transition

被引:33
|
作者
Li, Hui [1 ]
Li, Min [1 ]
Xu, Ding [1 ]
Zhao, Chun [1 ]
Liu, Guodong [1 ]
Wang, Fang [1 ]
机构
[1] Tongji Univ, Shanghai Peoples Hosp 10, Dept Ophthalmol, Shanghai 200072, Peoples R China
基金
中国国家自然科学基金;
关键词
Snail; Epithelial-mesenchymal transition; Retinal pigment epithelial; Proliferative vitreoretinopathy; PROLIFERATIVE VITREORETINOPATHY; TUMOR PROGRESSION; CELLS; EXPRESSION; CANCER;
D O I
10.1016/j.bbrc.2014.02.119
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Snail transcription factor has been implicated as an important regulator in epithelial-mesenchymal transition (EMT) during tumourigenesis and fibrogenesis. Our previous work showed that Snail transcription factor was activated in transforming growth factor beta 1 (TGF-beta 1) induced EMT in retinal pigment epithelial (RPE) cells and may contribute to the development of retinal fibrotic disease such as proliferative vitreoretinopathy (PVR). However, whether Snail alone has a direct role on retinal pigment epithelial-mesenchymal transition has not been investigated. Here, we analyzed the capacity of Snail to drive EMT in human RPE cells. A vector encoding Snail gene or an empty vector were transfected into human RPE cell lines ARPE-19 respectively. Snail overexpression in ARPE-19 cells resulted in EMT, which was characterized by the expected phenotypic transition from a typical epithelial morphology to mesenchymal spindle-shaped. The expression of epithelial markers E-cadherin and Zona occludin-1 (Z0-1) were down-regulated, whereas mesenchymal markers a-smooth muscle actin (a-SMA) and fibronectin were up-regulated in Snail expression vector transfected cells. In addition, ectopic expression of Snail significantly enhanced ARPE-19 cell motility and migration. The present data suggest that overexpression of Snail in ARPE-19 cells could directly trigger EMT. These results may provide novel insight into understanding the regulator role of Snail in the development of retinal pigment epithelial-mesenchymal transition. (c) 2014 The Authors. Published by Elsevier Inc.
引用
收藏
页码:347 / 351
页数:5
相关论文
共 50 条
  • [21] The role of mechanical stretch and TGF-β2 in epithelial-mesenchymal transition of retinal pigment epithelial cells
    Qian Cao
    Qu-Zhen Deji
    Ya-Jun Liu
    Wei Ye
    Wang-Dui Zhaba
    Qin Jiang
    Feng Yan
    International Journal of Ophthalmology, 2019, 12 (12) : 1832 - 1838
  • [22] The role of mechanical stretch and TGF-β2 in epithelial-mesenchymal transition of retinal pigment epithelial cells
    Cao, Qian
    Deji, Qu-Zhen
    Liu, Ya-Jun
    Ye, Wei
    Zhaba, Wang-Dui
    Jiang, Qin
    Yan, Feng
    INTERNATIONAL JOURNAL OF OPHTHALMOLOGY, 2019, 12 (12) : 1832 - 1838
  • [23] Curcumin Inhibits Proliferation and Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells Via Multiple Pathways
    Zhou, X.
    Kuang, X.
    Long, C.
    Liu, W.
    Tang, Y.
    Liu, L.
    Liu, H.
    He, J.
    Huang, Z.
    Fan, Y.
    Zhang, Q.
    Shen, H.
    CURRENT MOLECULAR MEDICINE, 2017, 17 (04) : 312 - 319
  • [24] Epithelial-mesenchymal transition suppresses ERas to activate autophagy in retinal pigment epithelial cells in proliferative vitreoretinopathy
    Pan, J.
    Zhao, L-X
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2020, 24 (09) : 4627 - 4634
  • [25] Exosomes mediate an epithelial-mesenchymal transition cascade in retinal pigment epithelial cells: Implications for proliferative vitreoretinopathy
    Zhang, Yao
    Wang, Kaizhe
    Pan, Jiabin
    Yang, Shuai
    Yao, Haipei
    Li, Min
    Li, Hui
    Lei, Hetian
    Jin, Haiying
    Wang, Fang
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020, 24 (22) : 13324 - 13335
  • [26] Fucoidan Inhibits Epithelial-Mesenchymal Transition of Retinal Pigment Epithelium in Proliferative Vitreoretinopathy
    Zhang, Yao
    Li, Hui
    Yang, Shuai
    Yao, Haipei
    Zhang, Jingfa
    Xu, Guo-Tong
    Wang, Fang
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2017, 58 (08)
  • [27] Decorin for choroidal neovascularization fibrosis and epithelial-mesenchymal transition of retinal pigment epithelium
    Behar-Cohen, Francine F.
    Benichou, Laura
    Debillon, Louis
    Bigot, Karine
    Bordet, Thierry
    Zhao, Min
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2022, 63 (07)
  • [28] Inhibition of epithelial-mesenchymal transition in retinal pigment epithelial cells by a retinoic acid receptor-α agonist
    Kobayashi, Yuka
    Tokuda, Kazuhiro
    Yamashiro, Chiemi
    Higashijima, Fumiaki
    Yoshimoto, Takuya
    Ota, Manami
    Ogata, Tadahiko
    Ashimori, Atsushige
    Hatano, Makoto
    Kobayashi, Masaaki
    Uchi, Sho-Hei
    Wakuta, Makiko
    Kimura, Kazuhiro
    SCIENTIFIC REPORTS, 2021, 11 (01)
  • [29] Protective Effects of Fucoidan on Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells and Progression of Proliferative Vitreoretinopathy
    Zhang, Yao
    Zhao, Dongwan
    Yang, Shuai
    Yao, Haipei
    Li, Min
    Zhao, Chun
    Zhang, Jingfa
    Xu, Guo-Tong
    Li, Hui
    Wang, Fang
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 46 (04) : 1704 - 1715
  • [30] Epithelial-mesenchymal transition in breast epithelial cells treated with cadmium and the role of Snail
    Wei, Zhengxi
    Shan, Zhongguo
    Shaikh, Zahir A.
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2018, 344 : 46 - 55