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Overexpression of Snail in retinal pigment epithelial triggered epithelial-mesenchymal transition
被引:33
|作者:
Li, Hui
[1
]
Li, Min
[1
]
Xu, Ding
[1
]
Zhao, Chun
[1
]
Liu, Guodong
[1
]
Wang, Fang
[1
]
机构:
[1] Tongji Univ, Shanghai Peoples Hosp 10, Dept Ophthalmol, Shanghai 200072, Peoples R China
基金:
中国国家自然科学基金;
关键词:
Snail;
Epithelial-mesenchymal transition;
Retinal pigment epithelial;
Proliferative vitreoretinopathy;
PROLIFERATIVE VITREORETINOPATHY;
TUMOR PROGRESSION;
CELLS;
EXPRESSION;
CANCER;
D O I:
10.1016/j.bbrc.2014.02.119
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Snail transcription factor has been implicated as an important regulator in epithelial-mesenchymal transition (EMT) during tumourigenesis and fibrogenesis. Our previous work showed that Snail transcription factor was activated in transforming growth factor beta 1 (TGF-beta 1) induced EMT in retinal pigment epithelial (RPE) cells and may contribute to the development of retinal fibrotic disease such as proliferative vitreoretinopathy (PVR). However, whether Snail alone has a direct role on retinal pigment epithelial-mesenchymal transition has not been investigated. Here, we analyzed the capacity of Snail to drive EMT in human RPE cells. A vector encoding Snail gene or an empty vector were transfected into human RPE cell lines ARPE-19 respectively. Snail overexpression in ARPE-19 cells resulted in EMT, which was characterized by the expected phenotypic transition from a typical epithelial morphology to mesenchymal spindle-shaped. The expression of epithelial markers E-cadherin and Zona occludin-1 (Z0-1) were down-regulated, whereas mesenchymal markers a-smooth muscle actin (a-SMA) and fibronectin were up-regulated in Snail expression vector transfected cells. In addition, ectopic expression of Snail significantly enhanced ARPE-19 cell motility and migration. The present data suggest that overexpression of Snail in ARPE-19 cells could directly trigger EMT. These results may provide novel insight into understanding the regulator role of Snail in the development of retinal pigment epithelial-mesenchymal transition. (c) 2014 The Authors. Published by Elsevier Inc.
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页码:347 / 351
页数:5
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