Visual detection of microRNA with lateral flow nucleic acid biosensor

被引：125

作者：

Gao, Xuefei ^{[1
,2
]}

Xu, Hui ^{[2
]}

Baloda, Meenu ^{[2
]}

Gurung, Anant S. ^{[2
]}

Xu, Li-Ping ^{[1
]}

Wang, Tao ^{[2
]}

Zhang, Xueji ^{[1
]}

Liu, Guodong ^{[2
,3
]}

机构：

[1] Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China

[2] N Dakota State Univ, Dept Chem & Biochem, Fargo, ND 58105 USA

[3] Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 35002, Fujian, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2014年 / 54卷

基金：

北京市自然科学基金;

关键词：

MicroRNA; Lateral flow biosensor; Nucleic acid; Visual detection; P53-INDUCIBLE MICRORNAS; ESCHERICHIA-COLI; EXPRESSION; NANOPARTICLES; STRIP;

D O I：

10.1016/j.bios.2013.10.055

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report a DNA-gold nanoparticle (DNA-GNP) based lateral flow nucleic acid biosensor for visual detection of microRNA (miRNA)-215 in aqueous solutions and biological samples with low-cost and short analysis time. Sandwich-type hybridization reactions among GNP-labeled DNA probe, miRNA-215 and biotin-modified DNA probes were performed on the lateral flow device. The accumulation of GNPs on the test zone of the biosensor enables the visual detection of miRNA-215. After systematic optimization, the biosensor was able to detect a minimum concentration of 60 pM miRNA-215. The biosensor was applied to detect miRNA-215 from A549 cell lysate directly without complex sample treatment, and the detection limit of 0.148 million cells was obtained. This study provides a simple, rapid, specific and low-cost approach for miRNA detection in aqueous solutions and biological samples, showing great promise for clinical application and biomedical diagnosis in some malignant diseases. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：578 / 584

页数：7

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