Connexin 48.5 is required for normal cardiovascular function and lens development in zebrafish embryos

被引:24
作者
Cheng, SH
Shakespeare, T
Mui, R
White, TW
Valdimarsson, G [1 ]
机构
[1] Univ Manitoba, Dept Zool, Winnipeg, MB R3T 2N2, Canada
[2] Univ Manitoba, Dept Biochem & Med Genet, Winnipeg, MB R3T 2N2, Canada
[3] SUNY Stony Brook, Dept Physiol & Biophys, Stony Brook, NY 11794 USA
关键词
D O I
10.1074/jbc.M401355200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gap junctions are composed of connexin (Cx) proteins and mediate intercellular communication required for many developmental and physiological processes. Here we describe the isolation and characterization of Cx48.5, a zebrafish connexin with the highest sequence identity to mammalian Cx46. Expression analysis showed that Cx48.5 is expressed in the adult and embryonic lens and heart, adult testis, and transiently in the embryonic otic vesicles. Injection of Cx48.5 cRNA into Xenopus oocytes elicited intercellular electrical coupling with voltage sensitivity similar to mammalian Cx46. In single oocytes, Cx48.5 also induced large outward currents on depolarization, consistent with gap-junctional hemichannels. Disruption of Cx48.5 expression in embryos with antisense morpholino oligos (morpholinos) revealed that Cx48.5 has an essential role in the maintenance of lens homeostasis. The morpholino-treated embryos also developed small lenses and eyes as well as severe cardiovascular abnormalities.
引用
收藏
页码:36993 / 37003
页数:11
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