In search of an "autophagomometer"

被引：487

作者：

Rubinsztein, David C. ^{[1
]}

Cuervo, Ana Maria ^{[2
]}

Ravikumar, Brinda

Sarkar, Sovan

Korolchuk, Viktor

Kaushik, Susmita ^{[2
]}

Klionsky, Daniel J. ^{[3
,4
,5
]}

机构：

[1] Univ Cambridge, Cambridge Inst Med Res, Addenbrookes Hosp, Dept Med Genet, Cambridge CB2 2XY, Cambs, England

[2] Albert Einstein Coll Med, Inst Aging Res, Marion Bessin Liver Res Ctr, Dept Dev & Mol Biol, Bronx, NY 10467 USA

[3] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA

[4] Univ Michigan, Dept Biol Chem, Ann Arbor, MI 48109 USA

[5] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA

来源：

AUTOPHAGY | 2009年 / 5卷 / 05期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

autophagy; flux; synthesis; LC3; assay; AUTOPHAGY; LYSOSOMES; FUSION; LC3; DEGRADATION; MATURATION; INHIBITION; CLEARANCE; PROTEIN; PATHWAY;

D O I：

10.4161/auto.5.5.8823

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent years have seen the realization that macroautophagy (which we will call autophagy) is not only important in yeast but is necessary for diverse functions in plants and animals. Importantly, autophagy can have an impact on human pathologies including infectious diseases, cancers, and neurodegenerative conditions. Thus, we need to be able to measure autophagy accurately in order to understand how it can be regulated physiologically and with exogenous agents.

引用

页码：585 / 589

页数：5

共 17 条

[1] Microtubules support production of starvation-induced autophagosomes but not their targeting and fusion with lysosomes [J].

Fass, Ephraim ;

Shvets, Elena ;

Degani, Ilan ;

Hirschberg, Koret ;

Elazar, Zvulun .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (47) :36303-36316

[2]

GAMERDINGER M, 2009, EMBO J

[3] The itinerary of autophagosomes: From peripheral formation to kiss-and-run fusion with lysosomes [J].

Jahreiss, Luca ;

Menzies, Fiona M. ;

Rubinsztein, David C. .

TRAFFIC, 2008, 9 (04) :574-587

[4] LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing [J].

Kabeya, Y ;

Mizushima, N ;

Uero, T ;

Yamamoto, A ;

Kirisako, T ;

Noda, T ;

Kominami, E ;

Ohsumi, Y ;

Yoshimori, T .

EMBO JOURNAL, 2000, 19 (21) :5720-5728

[5] Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3 [J].

Kimura, Shunsuke ;

Noda, Takeshi ;

Yoshimori, Tamotsu .

AUTOPHAGY, 2007, 3 (05) :452-460

[6] Does bafilomycin A1 block the fusion of autophagosomes with lysosomes? [J].

Klionsky, Daniel J. ;

Elazar, Zvulun ;

Seglen, Per O. ;

Rubinsztein, David C. .

AUTOPHAGY, 2008, 4 (07) :849-850

[7] Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes [J].

Klionsky, Daniel J. ;

Abeliovich, Hagai ;

Agostinis, Patrizia ;

Agrawal, Devendra K. ;

Aliev, Giumrakch ;

Askew, David S. ;

Baba, Misuzu ;

Baehrecke, Eric H. ;

Bahr, Ben A. ;

Ballabio, Andrea ;

Bamber, Bruce A. ;

Bassham, Diane C. ;

Bergamini, Ettore ;

Bi, Xiaoning ;

Biard-Piechaczyk, Martine ;

Blum, Janice S. ;

Breclesen, Dale E. ;

Brodsky, Jeffrey L. ;

Brumell, John H. ;

Brunk, Ulf T. ;

Bursch, Wilfried ;

Camougrand, Nadine ;

Cebollero, Eduardo ;

Cecconi, Francesco ;

Chen, Yingyu ;

Chin, Lih-Shen ;

Choi, Augustine ;

Chu, Charleen T. ;

Chung, Jongkyeong ;

Clarke, Peter G. H. ;

Clark, Robert S. B. ;

Clarke, Steven G. ;

Clave, Corinne ;

Cleveland, John L. ;

Codogno, Patrice ;

Colombo, Maria I. ;

Coto-Montes, Ana ;

Cregg, James M. ;

Cuervo, Ana Maria ;

Debnath, Jayanta ;

Demarchi, Francesca ;

Dennis, Patrick B. ;

Dennis, Phillip A. ;

Deretic, Vojo ;

Devenish, Rodney J. ;

Di Sano, Federica ;

Dice, J. Fred ;

DiFiglia, Marian ;

Dinesh-Kumar, Savithramma ;

Distelhorst, Clark W. .

AUTOPHAGY, 2008, 4 (02) :151-175

[8] Autophagy Inhibition Compromises Degradation of Ubiquitin-Proteasome Pathway Substrates [J].

Korolchuk, Viktor I. ;

Mansilla, Alicia ;

Menzies, Fiona M. ;

Rubinsztein, David C. .

MOLECULAR CELL, 2009, 33 (04) :517-527

[9] How to interpret LC3 immunoblotting [J].

Mizushima, Noboru ;

Yoshimori, Tamotsu .

AUTOPHAGY, 2007, 3 (06) :542-545

[10] Potential therapeutic applications of autophagy [J].

Rubinsztein, David C. ;

Gestwicki, Jason E. ;

Murphy, Leon O. ;

Klionsky, Daniel J. .

NATURE REVIEWS DRUG DISCOVERY, 2007, 6 (04) :304-312

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