In search of an "autophagomometer"

被引：487
作者：
Rubinsztein, David C. [1 ]
Cuervo, Ana Maria [2 ]
Ravikumar, Brinda
Sarkar, Sovan
Korolchuk, Viktor
Kaushik, Susmita [2 ]
Klionsky, Daniel J. [3 ,4 ,5 ]
机构：
[1] Univ Cambridge, Cambridge Inst Med Res, Addenbrookes Hosp, Dept Med Genet, Cambridge CB2 2XY, Cambs, England
[2] Albert Einstein Coll Med, Inst Aging Res, Marion Bessin Liver Res Ctr, Dept Dev & Mol Biol, Bronx, NY 10467 USA
[3] Univ Michigan, Dept Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Biol Chem, Ann Arbor, MI 48109 USA
[5] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA
来源：
AUTOPHAGY | 2009年 / 5卷 / 05期
基金：
英国医学研究理事会; 英国惠康基金;
关键词：
autophagy; flux; synthesis; LC3; assay; AUTOPHAGY; LYSOSOMES; FUSION; LC3; DEGRADATION; MATURATION; INHIBITION; CLEARANCE; PROTEIN; PATHWAY;
D O I：
10.4161/auto.5.5.8823
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Recent years have seen the realization that macroautophagy (which we will call autophagy) is not only important in yeast but is necessary for diverse functions in plants and animals. Importantly, autophagy can have an impact on human pathologies including infectious diseases, cancers, and neurodegenerative conditions. Thus, we need to be able to measure autophagy accurately in order to understand how it can be regulated physiologically and with exogenous agents.
引用
收藏
页码：585 / 589
页数：5
相关论文
共 17 条
  • [1] Microtubules support production of starvation-induced autophagosomes but not their targeting and fusion with lysosomes
    Fass, Ephraim
    Shvets, Elena
    Degani, Ilan
    Hirschberg, Koret
    Elazar, Zvulun
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (47) : 36303 - 36316
  • [2] GAMERDINGER M, 2009, EMBO J
  • [3] The itinerary of autophagosomes: From peripheral formation to kiss-and-run fusion with lysosomes
    Jahreiss, Luca
    Menzies, Fiona M.
    Rubinsztein, David C.
    [J]. TRAFFIC, 2008, 9 (04) : 574 - 587
  • [4] LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
    Kabeya, Y
    Mizushima, N
    Uero, T
    Yamamoto, A
    Kirisako, T
    Noda, T
    Kominami, E
    Ohsumi, Y
    Yoshimori, T
    [J]. EMBO JOURNAL, 2000, 19 (21) : 5720 - 5728
  • [5] Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3
    Kimura, Shunsuke
    Noda, Takeshi
    Yoshimori, Tamotsu
    [J]. AUTOPHAGY, 2007, 3 (05) : 452 - 460
  • [6] Does bafilomycin A1 block the fusion of autophagosomes with lysosomes?
    Klionsky, Daniel J.
    Elazar, Zvulun
    Seglen, Per O.
    Rubinsztein, David C.
    [J]. AUTOPHAGY, 2008, 4 (07) : 849 - 850
  • [7] Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
    Klionsky, Daniel J.
    Abeliovich, Hagai
    Agostinis, Patrizia
    Agrawal, Devendra K.
    Aliev, Giumrakch
    Askew, David S.
    Baba, Misuzu
    Baehrecke, Eric H.
    Bahr, Ben A.
    Ballabio, Andrea
    Bamber, Bruce A.
    Bassham, Diane C.
    Bergamini, Ettore
    Bi, Xiaoning
    Biard-Piechaczyk, Martine
    Blum, Janice S.
    Breclesen, Dale E.
    Brodsky, Jeffrey L.
    Brumell, John H.
    Brunk, Ulf T.
    Bursch, Wilfried
    Camougrand, Nadine
    Cebollero, Eduardo
    Cecconi, Francesco
    Chen, Yingyu
    Chin, Lih-Shen
    Choi, Augustine
    Chu, Charleen T.
    Chung, Jongkyeong
    Clarke, Peter G. H.
    Clark, Robert S. B.
    Clarke, Steven G.
    Clave, Corinne
    Cleveland, John L.
    Codogno, Patrice
    Colombo, Maria I.
    Coto-Montes, Ana
    Cregg, James M.
    Cuervo, Ana Maria
    Debnath, Jayanta
    Demarchi, Francesca
    Dennis, Patrick B.
    Dennis, Phillip A.
    Deretic, Vojo
    Devenish, Rodney J.
    Di Sano, Federica
    Dice, J. Fred
    DiFiglia, Marian
    Dinesh-Kumar, Savithramma
    Distelhorst, Clark W.
    [J]. AUTOPHAGY, 2008, 4 (02) : 151 - 175
  • [8] Autophagy Inhibition Compromises Degradation of Ubiquitin-Proteasome Pathway Substrates
    Korolchuk, Viktor I.
    Mansilla, Alicia
    Menzies, Fiona M.
    Rubinsztein, David C.
    [J]. MOLECULAR CELL, 2009, 33 (04) : 517 - 527
  • [9] How to interpret LC3 immunoblotting
    Mizushima, Noboru
    Yoshimori, Tamotsu
    [J]. AUTOPHAGY, 2007, 3 (06) : 542 - 545
  • [10] Potential therapeutic applications of autophagy
    Rubinsztein, David C.
    Gestwicki, Jason E.
    Murphy, Leon O.
    Klionsky, Daniel J.
    [J]. NATURE REVIEWS DRUG DISCOVERY, 2007, 6 (04) : 304 - 312
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