Time-Dependent Decrease of Clusterin as a Potential Cerebrospinal Fluid Biomarker for Drug-Resistant Epilepsy

被引：18
作者：
Yu, Weihua [1 ]
Chen, Dan [2 ]
Wang, Zhihua [2 ]
Zhou, Chunlei [2 ]
Luo, Jing [2 ]
Xu, Yali [2 ]
Shen, Lan [2 ]
Yin, Huan [2 ]
Tao, Shuxin [2 ]
Xiao, Zheng [2 ]
Xiao, Fei [2 ]
Lu, Yang [3 ]
Wang, Xuefeng [2 ]
机构：
[1] Chongqing Med Univ, Inst Neurosci, Chongqing 400016, Peoples R China
[2] Chongqing Med Univ, Affiliated Hosp 1, Dept Neurol, Chongqing 400016, Peoples R China
[3] Chongqing Med Univ, Affiliated Hosp 1, Dept Geriatr, Chongqing 400016, Peoples R China
来源：
JOURNAL OF MOLECULAR NEUROSCIENCE | 2014年 / 54卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Epilepsy; Clusterin; Cerebrospinal fluid; Rat model; Resistance; SULFATED GLYCOPROTEIN-2; NEUROLOGICAL DISORDERS; SEIZURE; APOPTOSIS; BARRIER; PROTEIN;
D O I：
10.1007/s12031-014-0237-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Our previous study on proteomic analysis has shown that clusterin (CLU) is significantly decreased in the cerebrospinal fluid (CSF) of patients with epilepsy. Therefore, the present study aimed to confirm CLU concentration reduction in the CSF of patients with drug-resistant epilepsy and drug-responsive epilepsy. Fifty-two patients with epilepsy (23 drug resistance and 29 drug effectivity) and 20 control individuals were recruited. The concentrations of CSF and serum CLU were detected. Moreover, alteration of CLU was detected in the rat hippocampus over time after pilocarpine-induced status epilepticus (SE). Our results showed that human CSF-CLU levels were decreased in patients with both drug-resistant epilepsy and drug-responsive epilepsy compared to controls, and concentration of CSF-CLU was obviously lower in drug-resistant epilepsy than in drug-responsive epilepsy. In the pilocarpine-induced seizure rats, expression of neuronal CLU was gradually decreased in a time-dependent manner from acute phase to chronic phase after the onset of SE. In conclusion, CLU level is decreased in the CSF of human epilepsy and the similar alteration is confirmed in a rat model with epilepsy. Therefore, CLU might contribute to the development of epilepsy and be a potential CSF biomarker for resistant epilepsy.
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页码：1 / 9
页数：9
相关论文
共 29 条
[1]   Nuclear CLU (nCLU) and the Fate of the Cell [J].
Bettuzzi, Saverio ;
Rizzi, Federica .
ADVANCES IN CANCER RESEARCH, VOL 104, 2009, 104 :59-+
[2]   Advances in CANCER RESEARCH Volume 104 Clusterin, Part A Introduction [J].
Bettuzzi, Saverio .
ADVANCES IN CANCER RESEARCH, VOL 104, 2009, 104 :1-8
[3]  
BLASCHUK O, 1983, J BIOL CHEM, V258, P7714
[4]   Clusterin in neurological disorders: Molecular perspectives and clinical relevance [J].
Charnay, Yves ;
Imhof, Anouk ;
Vallet, Philippe G. ;
Kovari, Eniko ;
Bouras, Constantin ;
Giannakopoulos, Panteleimon .
BRAIN RESEARCH BULLETIN, 2012, 88 (05) :434-443
[5]   CLUSTERIN ACCUMULATES IN DYING NEURONS FOLLOWING STATUS EPILEPTICUS [J].
DRAGUNOW, M ;
PRESTON, K ;
DODD, J ;
YOUNG, D ;
LAWLOR, P ;
CHRISTIE, D .
MOLECULAR BRAIN RESEARCH, 1995, 32 (02) :279-290
[6]   CHOP regulates the p53-MDM2 axis and is required for neuronal survival after seizures [J].
Engel, Tobias ;
Sanz-Rodgriguez, Amaya ;
Jimenez-Mateos, Eva M. ;
Concannon, Caoimhin G. ;
Jimenez-Pacheco, Alba ;
Moran, Catherine ;
Mesuret, Guillaume ;
Petit, Emilie ;
Delanty, Norman ;
Farrell, Michael A. ;
O'Brien, Donncha F. ;
Prehn, Jochen H. M. ;
Lucas, Jose J. ;
Henshall, David C. .
BRAIN, 2013, 136 :577-592
[7]   RAM RETE TESTIS FLUID CONTAINS A PROTEIN (CLUSTERIN) WHICH INFLUENCES CELL-CELL INTERACTIONS INVITRO [J].
FRITZ, IB ;
BURDZY, K ;
SETCHELL, B ;
BLASCHUK, O .
BIOLOGY OF REPRODUCTION, 1983, 28 (05) :1173-1188
[8]   Contribution of apoptosis-associated signaling pathways to epileptogenesis: lessons from Bcl-2 family knockouts [J].
Henshall, David C. ;
Engel, Tobias .
FRONTIERS IN CELLULAR NEUROSCIENCE, 2013, 7
[9]   Protective Effect of Clusterin on Blood-Retinal Barrier Breakdown in Diabetic Retinopathy [J].
Kim, Jeong-Hun ;
Kim, Jin Hyoung ;
Yu, Young Suk ;
Min, Bon Hong ;
Kim, Kyu-Won .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2010, 51 (03) :1659-1665
[10]   Proapoptotic role of nuclear clusterin in brain [J].
Kim, Nayoung ;
Choi, Wan Sung .
ANATOMY & CELL BIOLOGY, 2011, 44 (03) :169-175
123