Proliferation, Differentiation and Immunoregulatory Capacities of Brown and White Adipose-Derived Stem Cells from Young and Aged Mice

被引:2
作者
Yang, Yan-Mei [1 ]
Dong, Xiao-Hui [2 ]
Ma, Wei-Chao [2 ]
Guan, Li-Hui [3 ]
Wang, Yu-Han [2 ]
Huang, Xiao-Hui [2 ]
Chen, Jun-Feng [1 ]
Zhao, Xin [2 ]
Yang, Shi-Ming [4 ]
Jiang, Xiao-Xia [2 ]
Wen, Ning [1 ]
机构
[1] Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Stomatol, 28 Fuzing Rd, Beijing 100853, Peoples R China
[2] Acad Mil Med Sci, Inst Mil Cognit & Brain Sci, Dept Neural Engn & Biol Interdisciplinary Studies, 27 Taiping Rd, Beijing 100850, Peoples R China
[3] Hebei North Univ, Coll Anim Sci & Technol, Dept Anim Husb Engn, Zhangjiakou, Peoples R China
[4] Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China
基金
中国国家自然科学基金;
关键词
Brown adipose derived stem cells; White adipose derived stern cells; Differentiation; Immunoregulation; Aged mice; IN-VITRO; HARVESTING SITE; TISSUE; REGENERATION; SUPPRESSION; INHIBIT;
D O I
10.15283/ijsc20019
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Background and Objectives: Adipose tissue is a source of mesenchymal stem cells, which have the potential to differentiate into various types of cells. Adipose-derived stem cells (ADSCS) are now recognized as an accessible, abundant, and reliable stem cells suitable for tissue engineering and regenerative medicine applications. However, few literatures gave a comprehensive report on the capacities of ADSCs harvested from different sites. Especially, the capacities of ADSCs from aged mice remained unclear. In this study, we investigated several main capacities of brown adipose derived stem cells (B-ADSCs) and white adipose derived stem cells (W-ADSCs)from both young and aged mice. Methods and Results: When isolated from young mice, B-ADSCs showed a stronger proliferation rate and higher osteogenic, adipogenic and myocardial differentiation ability than W-ADSCs. Carbon , fluorescein diacetate succinimidyl ester (CFSE) labeling test suggested no significant difference in immunosuppression capacity between B-ADSCs and W-ADSCs. Similarly, no difference between these two were found in several immune related molecules, such as programmed death-ligand 1 (PD-1,1), intercellular cell adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), inducible nitric oxide synthase (iNOS), tumour necrosis factor-alpha (INF-alpha), interkukin 10 (IL10), and suppressor of cytokine signaling 1 (socsl). When isolated from aged mice, B-ADSCs also showed a stronger proliferation rate and higher osteogenic, adipogenic and myocardial differentiation ability than W-ADSCs; however, it demonstrated an attenuated immunosuppression capacity compared to W-ADSCs. Conclusions: In summary, our data showed that ADSCs' characteristics were tissue source dependent and changed with age. It provided evidence for choosing the right tissue-specific ADSCs for clinical application and fundamental research.
引用
收藏
页码:246 / 256
页数:11
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