Antagonism of Bacillus spp. Isolated from Marine Biofilms Against Terrestrial Phytopathogenic Fungi

被引:14
作者
Ortega-Morales, B. O. [1 ]
Ortega-Morales, F. N. [2 ]
Lara-Reyna, J. [2 ]
De la Rosa-Garcia, S. C. [1 ]
Martinez-Hernandez, A. [2 ]
Montero-M., Jorge [3 ]
机构
[1] Univ Autonoma Campeche, Ctr Invest Microbiol Ambiental & Biotecnol, Campeche 24030, Mexico
[2] Colegio Postgrad Campus Campeche, Campeche 24050, Mexico
[3] CINVESTAV, Dept Recursos Mar, Unidad Merida, Merida 97310, Yucatan, Mexico
关键词
Marine biofilm; Bacillus spp; Phytopathogens; Antagonism; Colonization; BIOLOGICAL-CONTROL; POSTHARVEST DISEASES; ITURIN-A; SUBTILIS; BACTERIA; FRUITS; SUPPRESSION; MOJAVENSIS; VEGETABLES; SURFACTIN;
D O I
10.1007/s10126-008-9152-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We aimed at determining the antagonistic behavior of bacteria derived from marine biofilms against terrestrial phytopathogenic fungi. Some bacteria closely related to Bacillus mojavensis (three isolates) and Bacillus firmus (one isolate) displayed antagonistic activity against Colletotrichum gloeosporioides ATCC 42374, selected as first screen organism. The four isolates were further quantitatively tested against C. gloeosporioides, Colletotrichum fragariae, and Fusarium oxysporum on two culture media, potato dextrose agar (PDA) and a marine medium-based agar [yeast extract agar (YEA)] at different times of growth of the antagonists (early, co-inoculation with the pathogen and late). Overall antagonistic assays showed differential susceptibility among the pathogens as a function of the type of culture media and time of colonization (P < 0.05). In general, higher suppressive activities were recorded for assays performed on YEA than on PDA; and also when the antagonists were allowed to grow 24 h earlier than the pathogen. F. oxysporum was the most resistant fungus while the most sensitive was C. gloeosporioides ATCC 42374. Significant differences in antagonistic activity (P < 0.05) were found between the different isolates. In general, Bacillus sp. MC3B-22 displayed a greater antagonistic effect than the commercial biocontrol strain Bacillus subtilis G03 (KodiakA (R)). Further incubation studies and scanning electronic microscopy revealed that Bacillus sp. MC3B-22 was able to colonize, multiply, and inhibit C. gloeosporioides ATCC 42374 when tested in a mango leaf assay, showing its potential for fungal biocontrol. Additional studies are required to definitively identify the active isolates and to determine their mode of antifungal action, safety, and biocompatibility.
引用
收藏
页码:375 / 383
页数:9
相关论文
共 50 条
[31]   Development of molecular typing methods for Bacillus spp. and Paenibacillus spp. isolated from fluid milk products [J].
Durak, MZ ;
Fromm, HI ;
Huck, JR ;
Zadoks, RN ;
Boor, KJ .
JOURNAL OF FOOD SCIENCE, 2006, 71 (02) :M50-M56
[32]   Antifungal activity of Bacillus velezensis and Pseudomonas azotoformans isolated from compost tea against anthracnose (Colletotrichum spp.) on strawberry fruit [J].
Popescu, Irina ;
Loganathan, A. Kiripuvaney ;
Graham, Hailey R. ;
Avis, Tyler J. .
PLANT PATHOLOGY, 2024, 73 (09) :2419-2428
[33]   Endophytic Bacillus spp. of coffee plants (Coffea arabica L.) and its potential in the biocontrol of phytopathogenic fungi and Lepidoptera larvae [J].
de Castro, Marcelo Tavares ;
Ferreira, Antonia Debora Camila de Lima ;
do Nascimento, Izabela Nunes ;
Rocha, Gabriela Teodoro ;
Celestino, Matheus Felipe ;
Freire, italo Alves ;
Moreira, Isabela Cardoso Farias ;
Gomes, Giovana Cidade ;
Cunha, Breno Beda dos Reis ;
Montalvao, Sandro Coelho Linhares ;
Moreira, Flavia Melo ;
Monnerat, Rose Gomes .
EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL, 2025, 35 (01)
[34]   Protease and γ-Glutamyltranspeptidase Activities of Bacillus spp. Isolated from Rice Straw [J].
Yang, Joo-Hee ;
Kim, In-Su ;
Oh, Chul-Hwan ;
Oh, Nam-Soon .
FOOD SCIENCE AND BIOTECHNOLOGY, 2012, 21 (01) :291-294
[35]   Biocontrol Activity of Bacillus spp. and Pseudomonas spp. Against Botrytis cinerea and Other Cannabis Fungal Pathogens [J].
Balthazar, Carole ;
Novinscak, Amy ;
Cantin, Gabrielle ;
Joly, David L. ;
Filion, Martin .
PHYTOPATHOLOGY, 2022, 112 (03) :549-560
[36]   In vitro Antagonism of Strains of Trichoderma spp., on Pathogenic Fungi of Nopal Vegetable [J].
Valencia de Ita, Ma Angeles ;
Parraguirre Lezama, Conrado ;
Baez Simon, Alfredo ;
Morales Mora, Luis A. ;
Huerta Lara, Manuel ;
Laug Garcia, Beatriz ;
Romero-Arenas, Omar .
JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2020, 14 (02) :1345-1352
[37]   Antibiofilm activity of amylase from Bacillus spp. against foodborne pathogens [J].
Mercimek, Takci Hatice Aysun ;
Pemra, Bakirhan ;
Esra, Colak ;
Mevlut, Saygin .
RESEARCH JOURNAL OF BIOTECHNOLOGY, 2021, 16 (05) :169-173
[38]   Profiles of Bacillus spp. Isolated from the Rhizosphere of Suaeda glauca and Their Potential to Promote Plant Growth and Suppress Fungal Phytopathogens [J].
Lu, Ping ;
Jiang, Ke ;
Hao, Ya-Qiao ;
Chu, Wan-Ying ;
Xu, Yu-Dong ;
Yang, Jia-Yao ;
Chen, Jia-Le ;
Zeng, Guo-Hong ;
Gu, Zhou-Hang ;
Zhao, Hong-Xin .
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2021, 31 (09) :1231-1240
[39]   Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis [J].
Sam-on, Muhamad Firdaus Syahmi ;
Mustafa, Shuhaimi ;
Yusof, Mohd Termizi ;
Hashim, Amalia Mohd ;
Abbasiliasi, Sahar ;
Zulkifly, Shahrizim ;
Jahari, Muhamad Amin ;
Roslan, Muhamad Akhmal Hakim .
MICROBIAL PATHOGENESIS, 2022, 164
[40]   Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia [J].
Alqahtani, Omaish ;
Stapleton, Paul ;
Gibbons, Simon .
SAUDI PHARMACEUTICAL JOURNAL, 2023, 31 (07) :1237-1243