An Amino Acid-Swapped Genetic Code

被引：15

作者：

Fujino, Tomoshige ^{[1
]}

Tozaki, Masahiro ^{[1
]}

Murakami, Hiroshi ^{[1
,2
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Dept Biomol Engn, Nagoya, Aichi 4648603, Japan

[2] Nagoya Univ, Inst NanoLife Syst, Inst Innovat Future Soc, Nagoya, Aichi 4648603, Japan

来源：

ACS SYNTHETIC BIOLOGY | 2020年 / 9卷 / 10期

基金：

日本学术振兴会;

关键词：

genetic code; cell-free translation; tRNA; aminoacyl-tRNA synthetase; genetic firewall; TRANSFER-RNA-SYNTHETASE; BIOLOGICAL CONTAINMENT; TRANSFER RNA(SER); IN-VIVO; ORGANISMS; GENOME; DISCRIMINATION; TRNA(GGC)(ALA); REASSIGNMENT; RECOGNITION;

D O I：

10.1021/acssynbio.0c00196

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Preventing the escape of hazardous genes from genetically modified organisms (GMOs) into the environment is one of the most important issues in biotechnology research. Various strategies were developed to create "genetic firewalls" that prevent the leakage of GMOs; however, they were not specially designed to prevent the escape of genes. To address this issue, we developed amino acid (AA)-swapped genetic codes orthogonal to the standard genetic code, namely SL (Ser and Leu were swapped) and SLA genetic codes (Ser, Leu, and Ala were swapped). From mRNAs encoded by the AA-swapped genetic codes, functional proteins were only synthesized in translation systems featuring the corresponding genetic codes. These results clearly demonstrated the orthogonality of the AA-swapped genetic codes against the standard genetic code and their potential to function as "genetic firewalls for genes". Furthermore, we propose "a codon-bypass strategy" to develop a GMO with an AA-swapped genetic code.

引用

页码：2703 / 2713

页数：11

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