Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis

被引:24
作者
Nakamura, Kodai [1 ]
Hiyake, Naomi [2 ]
Hamada, Tomofumi [1 ,3 ]
Yokoyama, Seiya [4 ]
Mori, Kazuki [1 ]
Yamashiro, Kouta [1 ]
Beppu, Mahiro [1 ]
Sagara, Yasuaki [5 ]
Sagara, Yoshiaki [6 ]
Sugiura, Tsuyoshi [1 ]
机构
[1] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Maxillofacial Diagnost & Surg Sci, Field Oral & Maxillofacial Rehabil, Kagoshima 8908544, Japan
[2] Kyushu Univ, Grad Sch Dent Sci, Dept Oral & Maxillofacial Surg, Div Maxillofacial Diagnost & Surg Sci, Fukuoka 8128582, Japan
[3] Hakuaikai Med Cooperat Sagara Hosp, Dept Oral & Maxillofacial Surg, Kagoshima 8920833, Japan
[4] Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Pathol, Kagoshima 8908544, Japan
[5] Hakuaikai Med Cooperat Sagara Hosp, Dept Breast Surg Oncol, Kagoshima 8920833, Japan
[6] Sagara Hosp Kagoshima, Dept Radiol, Hakuaikai Med Cooperat, Kagoshima 8920833, Japan
关键词
oral cancer; oral squamous cell carcinoma; microRNA; biomarker; tumor marker;
D O I
10.3390/cancers13030449
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Simple Summary Although early detection of oral squamous cell carcinoma (OSCC) is considered vital, classical biomarkers have shown poor sensitivity and specificity for early detection and monitoring of OSCC. Therefore, identification of reliable and sensitive biomarkers allowing for early detection and monitoring of OSCC is of the utmost importance. In this study, we successfully identified significantly upregulated or downregulated microRNAs in OSCC patients, and reported that a combination of six microRNAs could distinguish between OSCC and the control group with a higher degree of accuracy. Furthermore, compared with serum squamous cell carcinoma (SCC) antigen, the miRNA panel reflected the presence of OSCC accurately. The present results suggest that the combined microRNA panel based on serum microRNA levels shows potential as a novel diagnostic biomarker of OSCC. A lack of reliable biomarkers for oral squamous cell carcinoma (OSCC) poses a major clinical issue. The sensitivity and specificity of classical serum tumor markers, such as the squamous cell carcinoma antigen (SCC-Ag), are quite poor, especially for early detection. This study aimed to identify specific serum miRNAs potentially serving as OSCC biomarkers. The expression levels of candidate miRNAs in serum samples from 40 OSCC patients and 40 healthy controls were quantitatively analyzed via microarray and reverse transcription PCR (RT-PCR) analyses. To enhance the accuracy of detection, we used Fisher's linear discriminant analysis to establish a diagnostic model that incorporated a combination of selected miRNAs. Consequently, miR-19a and miR-20a were significantly upregulated in the patient group (p = 0.014 and 0.036, respectively), whereas miR-5100 was downregulated (p = 0.001). We found that a combination of six miRNAs (miR-24, miR-20a, miR-122, miR-150, miR-4419a, and miR-5100) could distinguish between OSCC and the control group with a higher degree of accuracy (Area Under the Curve, AUC: 0.844, sensitivity: 55%, and specificity: 92.5%). Furthermore, compared to serum SCC antigen, the 6-miRNA panel could accurately detect the presence of OSCC. The present specific miRNAs panel may serve as a novel candidate biomarker of oral cancer.
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页码:1 / 14
页数:14
相关论文
共 55 条
[1]   Galectin-1 and galectin-3: Plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [J].
Aggarwal, Sadhna ;
Sharma, Suresh C. ;
Das, Satya N. .
CLINICA CHIMICA ACTA, 2015, 442 :13-21
[2]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[3]   Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers [J].
Calin, GA ;
Sevignani, C ;
Dan Dumitru, C ;
Hyslop, T ;
Noch, E ;
Yendamuri, S ;
Shimizu, M ;
Rattan, S ;
Bullrich, F ;
Negrini, M ;
Croce, CM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (09) :2999-3004
[4]   MicroRNA signatures in human cancers [J].
Calin, George A. ;
Croce, Carlo M. .
NATURE REVIEWS CANCER, 2006, 6 (11) :857-866
[5]   Discovery of potential serum protein biomarkers for lymph node metastasis in oral cancer [J].
Chai, Yang D. ;
Zhang, Lifeng ;
Yang, Yan ;
Su, Trent ;
Charugundla, Prashant ;
Ai, Jiye ;
Messadi, Diana ;
Wong, David T. ;
Hu, Shen .
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 2016, 38 (01) :118-125
[6]   MicroRNA-17/20a functions to inhibit cell migration and can be used a prognostic marker in oral squamous cell carcinoma (vol 49, pg 923, 2013) [J].
Chang, Cheng-Chi ;
Yang, Yu-Jen ;
Li, Yue-Ju ;
Chen, Szu-Ta ;
Lin, Been-Ren ;
Wu, Tai-Sheng ;
Lin, Sze-Kwen ;
Kuo, Mark Yen-Ping ;
Tan, Ching-Ting .
ORAL ONCOLOGY, 2017, 72 :202-203
[7]   Using SCC Antigen and CRP Levels as Prognostic Biomarkers in Recurrent Oral Cavity Squamous Cell Carcinoma [J].
Chen, I-How ;
Liao, Chun-Ta ;
Wang, Hung-Ming ;
Huang, Jung-Ju ;
Kang, Chung-Jan ;
Huang, Shiang-Fu .
PLOS ONE, 2014, 9 (07)
[8]   Downregulation of miR-19a inhibits the proliferation and promotes the apoptosis of osteosarcoma cells by regulating the JAK2/STAT3 pathway [J].
Chen, Jiangqiang ;
Chen, Zuhui .
ONCOLOGY LETTERS, 2020, 20 (05)
[9]   Serum microRNA expression levels can predict lymph node metastasis in patients with early-stage cervical squamous cell carcinoma [J].
Chen, Junying ;
Yao, Desheng ;
Li, Yue ;
Chen, Hong ;
He, Chanjuan ;
Ding, Nan ;
Lu, Yan ;
Ou, Tingyu ;
Zhao, Shan ;
Li, Li ;
Long, Fengyi .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2013, 32 (03) :557-567
[10]   Overexpression of microRNA-5100 decreases the aggressive phenotype of pancreatic cancer cells by targeting PODXL [J].
Chijiiwa, Yoshiro ;
Moriyama, Taiki ;
Ohuchida, Kenoki ;
Nabae, Toshinaga ;
Ohtsuka, Takao ;
Miyasaka, Yoshihiro ;
Fujita, Hayato ;
Maeyama, Ryo ;
Manabe, Tatsuya ;
Abe, Atsushi ;
Mizuuchi, Yusuke ;
Oda, Yoshinao ;
Mizumoto, Kazuhiro ;
Nakamura, Masafumi .
INTERNATIONAL JOURNAL OF ONCOLOGY, 2016, 48 (04) :1688-1700