Biomolecular kinetics analysis using long-range surface plasmon waveguides

被引：19

作者：

Khodami, Maryam ^{[1
,2
]}

Berini, Pierre ^{[1
,2
,3
]}

机构：

[1] Univ Ottawa, Sch Elect Engn & Comp Sci, 800 King Edward Ave, Ottawa, ON K1N 6N5, Canada

[2] Univ Ottawa, Ctr Res Photon, 25 Templeton Ave, Ottawa, ON K1N 6N5, Canada

[3] Univ Ottawa, Dept Phys, 150 Louis Pasteur Ave, Ottawa, ON K1N 6N5, Canada

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 243卷

基金：

加拿大自然科学与工程研究理事会;

关键词：

Kinetics; Biosensors; LRSPP; SPR; BSA; Antibody; ANTIBODY-ANTIGEN INTERACTIONS; SEROTYPE-SPECIFIC DETECTION; DENGUE VIRUS; SEROLOGICAL DIAGNOSIS; RESONANCE; BIOSENSOR; INFECTION;

D O I：

10.1016/j.snb.2016.11.120

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel, cost effective, label free and real time biosensor based on straight gold waveguides supporting long range surface plasmon polaritons (LRSPPs) is used to measure the kinetics constants of protein-protein interactions. Bovine serum albumin (BSA) and anti-bovine albumin antibody (anti-BSA) produced in rabbit are used as the bio-pair for the purposes of demonstrating and investigating the extraction of binding kinetics. BSA protein is immobilized on the surface using thiol coupling. The output power of the system is recorded continuously over time, and then converted into surface mass concentration. The kinetics constants are extracted from binding curves using the integrated rate equation. Linear least squares and non-linear least squares analysis are employed to obtain the constants and the results are compared. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：114 / 120

页数：7

共 24 条

[1] Selective detection of bacteria in urine with a long-range surface plasmon waveguide biosensor [J].