Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp strain PCC6803

被引：76

作者：

Morohoshi, T

Maruo, T

Shirai, Y

Kato, J

Ikeda, T

Takiguchi, N

Ohtake, H

Kuroda, A ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Adv Sci Matter, Dept Mol Biotechnol, Hiroshima 7398530, Japan

[2] Japan Sci & Technol Corp, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 08期

关键词：

D O I：

10.1128/AEM.68.8.4107-4110.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The biological process for phosphate (Pi) removal is based on the use of bacteria capable of accumulating inorganic polyphosphate (polyP). We obtained Escherichia coli mutants which accumulate a large amount of polyP. The polyP accumulation in these mutants was ascribed to a mutation of the phoU gene that encodes a negative regulator of the Pi regulon. Insertional inactivation of the phoU gene also elevated the intracellular level of polyP in Synechocystis sp. strain PCC6803. The mutant could remove fourfold more P, from the medium than the wild-type strain removed.

引用

页码：4107 / 4110

页数：4