Structure-based design of peptides that self-assemble into regular polyhedral nanoparticles

被引:145
作者
Raman, Senthilkumar
Machaidze, Gia
Lustig, Ariel
Aebi, Ueli
Burkhard, Peter
机构
[1] M.E. Müller Institute for Structural Biology, Biozentrum, University of Basel, Basel
关键词
Antigen display; Drug delivery; Drug targeting; Peptide nanoparticles; Protein design;
D O I
10.1016/j.nano.2006.04.007
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Artificial particulate systems such as polymeric beads and liposomes are being applied in drug delivery, drug targeting, antigen display, vaccination, and other technologies. Here we used computer modeling to design a novel type of nanoparticles composed of peptides as building blocks. We verified the computer models via solid-phase peptide synthesis and biophysical analyses. We describe the structure-based design of a novel type of nanoparticles with regular polyhedral symmetry and a diameter of about 16 nm, which self-assembles from single polypeptide chains. Each peptide chain is composed of two coiled coil oligomerization domains with different oligomerization states joined by a short linker segment. In aqueous solution the peptides form nanoparticles of about 16 nm diameter. Such peptide nanoparticles are ideally suited for medical applications such as drug targeting and drug delivery systems, such as imaging devices, or they may be used for repetitive antigen display. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:95 / 102
页数:8
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