Gene expression signatures for identifying diffuse-type gastric cancer associated with epithelial-mesenchymal transition

被引：52

作者：

Tanabe, Shihori ^{[1
]}

Aoyagi, Kazuhiko ^{[2
]}

Yokozaki, Hiroshi ^{[3
]}

Sasaki, Hiroki ^{[2
]}

机构：

[1] Natl Inst Hlth Sci, Setagaya Ku, Div Safety Informat Drug Food & Chem, Tokyo 1588501, Japan

[2] Natl Canc Ctr, Res Inst, Dept Translat Oncol, Chuo Ku, Tokyo 1040045, Japan

[3] Kobe Univ, Grad Sch Med, Dept Pathol, Chuo Ku, Kobe, Hyogo 6500017, Japan

来源：

INTERNATIONAL JOURNAL OF ONCOLOGY | 2014年 / 44卷 / 06期

关键词：

epithelial-mesenchymal transition; microarray; mesenchymal stem cell; gastric cancer; gene expression; STEM-CELLS; HEDGEHOG; SOX2; TUMOR; LUNG;

D O I：

10.3892/ijo.2014.2387

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Epithelial-mesenchymal transition (EMT) is associated with tumor malignancy. The hedgehog-EMT pathway is preferentially activated in diffuse-type gastric cancer (GC) compared with intestinal-type GC; however, histological typing is currently the only method for distinguishing these two major types of GC. We compared the gene expression profiles of 12 bone marrow-derived mesenchymal stem cell cultures and 5 diffuse-type GC tissue samples. Numerous upregulated or downregulated genes were identified in diffuse-type GC, including CDH1,CDH2,VIM,WNT4 and WNT5. Among these genes, the mRNA ratio of CDH2 to CDH1 could distinguish the 15 diffuse-type GC samples from the 17 intestinal-type GC samples. Our results suggested that the mesenchymal features were more prominent in diffuse-type GC than in intestinal-type GC, but were weaker in diffuse-type GC than in mesenchymal stem cells. Diffuse-type GC that has undergone extensive EMT, which has a poor prognosis, can be identified by quantitative PCR analysis of only two genes.

引用

页码：1955 / 1970

页数：16

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