Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity

被引：20

作者：

Biswas, Suvendu ^{[1
]}

McCullough, Brandon S. ^{[1
]}

Ma, Elena S. ^{[1
]}

LaJoie, Dollie ^{[2
]}

Russell, Colin W. ^{[3
]}

Brown, D. Garrett ^{[3
]}

Round, June L. ^{[3
]}

Ullman, Katharine S. ^{[2
]}

Mulvey, Matthew A. ^{[3
]}

Barrios, Amy M. ^{[1
]}

机构：

[1] Univ Utah, Coll Pharm, Dept Med Chem, Salt Lake City, UT 84112 USA

[2] Univ Utah, Sch Med, Dept Oncol Sci, Salt Lake City, UT 84112 USA

[3] Univ Utah, Sch Med, Dept Pathol, Salt Lake City, UT 84112 USA

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 14期

基金：

美国国家科学基金会;

关键词：

THROUGHPUT SCREENING ASSAYS; STAPHYLOCOCCUS-AUREUS; ALKALINE-PHOSPHATASE; CANCER; SUBSTRATE; TARGETS; AUTOFLUORESCENCE; INHIBITION; DISORDERS; MECHANISM;

D O I：

10.1039/c6cc09204g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two resorufin-based substrates for protein tyrosine phosphatase (PTP) activity have been synthesized. These substrates provide sensitive fluorogenic readouts of PTP activity in vitro and in living cells at both acidic and neutral pH. In addition, the presence of the pathogenic bacteria Staphylococcus aureus was detected visually using a colorimetric readout.

引用

页码：2233 / 2236

页数：4

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