Early Erythrolysis in the Hematoma After Experimental Intracerebral Hemorrhage

被引:99
作者
Dang, Ge [1 ,2 ]
Yang, Yuefan [1 ,3 ]
Wu, Gang [1 ]
Hua, Ya [1 ]
Keep, Richard F. [1 ]
Xi, Guohua [1 ,4 ]
机构
[1] Univ Michigan, Dept Neurosurg, Ann Arbor, MI 48109 USA
[2] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Neurol, Guangzhou, Guangdong, Peoples R China
[3] Fourth Mil Med Univ, Xijing Hosp, Dept Neurosurg, Xian, Peoples R China
[4] Univ Michigan, R5018 BSRB, 109 Zina Pitcher Pl, Ann Arbor, MI 48109 USA
基金
美国国家卫生研究院;
关键词
Intracerebral hemorrhage; T2*magnetic resonance imaging; Hematoma; Erythrolysis; CD163; Hypertension; BRAIN-INJURY; NEURONAL DEATH; DEFEROXAMINE; INFLAMMATION; IRON; MECHANISMS; MEMBRANE; EDEMA; RATS; CT;
D O I
10.1007/s12975-016-0505-3
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Erythrolysis occurs in the clot after intracerebral hemorrhage (ICH), and the release of hemoglobin causes brain injury, but it is unclear when such lysis occurs. The present study examined early erythrolysis in rats. ICH rats had an intracaudate injection of 100 mu l autologous blood, and sham rats had a needle insertion. All rats had T2 and T2* magnetic response imaging (MRI) scanning, and brains were used for histology and CD163 (a hemoglobin scavenger receptor) and DARPP-32 (a neuronal marker) immunohistochemistry. There was marked heterogeneity within the hematoma on T2* MRI, with a hyperintense or isointense core and a hypointense periphery. Hematoxylin and eosin staining in the same animals showed significant erythrolysis in the core with the formation of erythrocyte ghosts. The degree of erythrolysis correlated with the severity of perihematomal neuronal loss. Perihematomal CD163 was increased by day 1 after ICH and may be involved in clearing hemoglobin caused by early hemolysis. Furthermore, ICH resulted in more severe erythrolysis, neuronal loss, and perihematomal CD163 upregulation in spontaneously hypertensive rats compared to Wistar-Kyoto rats. In conclusion, T2*MRI-detectable early erythrolysis occurred in the clot after ICH and activated CD163. Hypertension is associated with enhanced erythrolysis in the hematoma.
引用
收藏
页码:174 / 182
页数:9
相关论文
共 50 条
  • [31] Imaging and clinical prognostic indicators for early hematoma enlargement after spontaneous intracerebral hemorrhage
    Ji, Nan
    Lu, Jing Jing
    Zhao, Yuan Li
    Wang, Shuo
    Zhao, Ji Zong
    NEUROLOGICAL RESEARCH, 2009, 31 (04) : 362 - 366
  • [32] Iron Neurotoxicity and Protection by Deferoxamine in Intracerebral Hemorrhage
    Li, Zhe
    Liu, Yang
    Wei, Ruixue
    Khan, Suliman
    Zhang, Ruiyi
    Zhang, Yan
    Yong, Voon Wee
    Xue, Mengzhou
    FRONTIERS IN MOLECULAR NEUROSCIENCE, 2022, 15
  • [33] Gr1+ Macrophages and Dendritic Cells Dominate the Inflammatory Infiltrate 12 h After Experimental Intracerebral Hemorrhage
    Hammond, Matthew D.
    Ai, Youxi
    Sansing, Lauren H.
    TRANSLATIONAL STROKE RESEARCH, 2012, 3 : S125 - S131
  • [34] Perihematomal Edema After Spontaneous Intracerebral Hemorrhage
    Ironside, Natasha
    Chen, Ching-Jen
    Ding, Dale
    Mayer, Stephan A.
    Connolly, Edward Sander, Jr.
    STROKE, 2019, 50 (06) : 1626 - 1633
  • [35] The combination of deferoxamine and minocycline strengthens neuroprotective effect on acute intracerebral hemorrhage in rats
    Li, Zhe
    Liu, Yang
    Wei, Ruixue
    Khan, Suliman
    Xue, Mengzhou
    Yong, V. Wee
    NEUROLOGICAL RESEARCH, 2021, 43 (10) : 855 - 865
  • [36] Role of lipocalin-2 in brain injury after intracerebral hemorrhage
    Ni, Wei
    Zheng, Mingzhe
    Xi, Guohua
    Keep, Richard F.
    Hua, Ya
    JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2015, 35 (09) : 1454 - 1461
  • [37] The Critical Role of Erythrolysis and Microglia/Macrophages in Clot Resolution After Intracerebral Hemorrhage: A Review of the Mechanisms and Potential Therapeutic Targets
    Zheng, Yonghe
    Tan, Xiaoxiao
    Cao, Shenglong
    CELLULAR AND MOLECULAR NEUROBIOLOGY, 2023, 43 (01) : 59 - 67
  • [38] NEUROPROTECTIVE EFFECT OF ERYTHROPOIETIN AND DARBEPOETIN ALFA AFTER EXPERIMENTAL INTRACEREBRAL HEMORRHAGE
    Grasso, Giovanni
    Graziano, Francesca
    Sfacteria, Alessandra
    Carletti, Fabio
    Meli, Francesco
    Maugeri, Rosario
    Passalacqua, Marcello
    Certo, Francesco
    Fazio, Marco
    Buemi, Michele
    Iacopino, Domenico Gerardo
    NEUROSURGERY, 2009, 65 (04) : 763 - 769
  • [39] Immune changes in peripheral blood and hematoma of patients with intracerebral hemorrhage
    Jiang, Chao
    Wang, Yali
    Hu, Qiangfu
    Shou, Jixin
    Zhu, Li
    Tian, Ning
    Sun, Lu
    Luo, Huan
    Zuo, Fangfang
    Li, Fangfang
    Wang, Yingying
    Zhang, Jing
    Wang, Jiarui
    Wang, Jian
    Zhang, Jiewen
    FASEB JOURNAL, 2020, 34 (02) : 2774 - 2791
  • [40] Unconjugated bilirubin contributes to early inflammation and edema after intracerebral hemorrhage
    Loftspring, Matthew C.
    Johnson, Holly L.
    Feng, Rui
    Johnson, Aaron J.
    Clark, Joseph F.
    JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2011, 31 (04) : 1133 - 1142