Differential Increases in the Expression of Intermediate Filament Proteins and Concomitant Morphological Changes of Transdifferentiating Rat Hepatic Stellate Cells Observed In Vitro

被引：6

作者：

Mezaki, Yoshihiro ^{[1
]}

Morii, Mayako ^{[2
]}

Hebiguchi, Taku ^{[2
]}

Yoshikawa, Kiwamu ^{[1
]}

Yamaguchi, Noriko ^{[1
]}

Miura, Mitsutaka ^{[1
]}

Imai, Katsuyuki ^{[1
]}

Yoshino, Hiroaki

Senoo, Haruki ^{[1
]}

机构：

[1] Akita Univ, Dept Cell Biol & Morphol, Grad Sch Med, Akita 0108543, Japan

[2] Akita Univ, Grad Sch Med, Dept Pediat Surg, Akita 0108543, Japan

来源：

ACTA HISTOCHEMICA ET CYTOCHEMICA | 2013年 / 46卷 / 05期

基金：

日本学术振兴会;

关键词：

hepatic stellate cell; intermediate filament; vimentin; desmin; myofibroblast; FAT-STORING CELLS; FIBRILLARY ACIDIC PROTEIN; ITO-CELLS; LIVER; DESMIN; FIBROSIS; ACTIVATION; COLLAGEN; IDENTIFICATION; HETEROGENEITY;

D O I：

10.1267/ahc.13007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The Primary function of hepatic stellate cells (HSCs) is the storage of vitamin A. However, they are also responsible for liver fibrosis and are therapeutic targets for treatment of liver cirrhosis. Among the many molecular markers that define quiescent or activated states of HSCs, the characteristics of type III intermediate filaments are of particular interest. Whereas vimentin and desmin are upregulated in activated HSCs, glial fibrillary acidic protein is downregulated in activated HSCs. The functional differences between vimentin and desmin are poorly understood. By time-course quantifications of several molecular markers for HSC activation, we observed that the expression of vimentin preceded that of desmin during the transdifferentiation of HSCs. The immunoreactivity of vimentin in transdifferentiated HSCs was more intense in perinuclear regions compared to that of desmin. We propose that the delayed expression of desmin following the expression of vimentin and the peripheral localization of desmin compared to vimentin are both related to the more extended phenotype of transdifferentiating HSCs observed in vitro.

引用

页码：137 / 143

页数：7

共 26 条

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