One-step purification of xylanase from Melanocarpus albomyces and ethylene glycol as a novel soluble additive for enhancing its thermal stability

被引：3
|
作者：
Gupta, A
Roy, I
Khare, SK
Bisaria, VS
Gupta, MN
机构：
[1] Indian Inst Technol, Dept Chem, New Delhi 110016, India
[2] Indian Inst Technol, Dept Biochem Engn & Biotechnol, New Delhi 110016, India
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 23期
关键词：
enzyme thermostabilization; ethylene glycol; Melanocarpus albomyces; xylanase purification;
D O I：
10.1023/A:1021114831650
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Hydrophobic interaction chromatography with Phenyl Sepharose 6 Fast Flow resulted in 7-fold purification of xylanase activity from Melanocarpus albomyces with over 60% recovery of the activity. The purified preparation consisted of two major isoenzymes out of seven present in the organism. Ethylene glycol, used as the eluent, enhanced the thermal stability of the xylanase activity from a half-life of 2.2 h at 55 degreesC to almost 40 h.
引用
收藏
页码：2005 / 2009
页数：5
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