Redox hydrogel-based amperometric bienzyme electrodes for fish freshness monitoring

被引:73
作者
Niculescu, M [1 ]
Nistor, C [1 ]
Frébort, I [1 ]
Pec, P [1 ]
Mattiasson, B [1 ]
Csöregi, E [1 ]
机构
[1] Univ Lund, Dept Biotechnol, S-22100 Lund, Sweden
关键词
D O I
10.1021/ac990848+
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This work presents the design and optimization of amperometric biosensors for the determination of biogenic amines (e.g., histamine, putrescine, cadaverine, tyramine, cystamine, agmatine, spermidine), commonly present in food products, and their application for monitoring of freshness in fish samples. The biosensors were used as the working electrodes of a three-electrode electrochemical cell of wall-jet type, operated at -50 mV vs Ag/AgCl, in a now injection system. Two different bienzyme electrode designs were considered, one based on the two enzymes [a newly isolated and purified amine oxidase (AO) and horseradish peroxidase (HRP)] simply adsorbed onto graphite electrodes, and one when they were crosslinked to an Os-based redox polymer. The redox hydrogel-based biosensors showed better biosensors characteristics, i.e., sensitivity of 0.194 A M-1 cm(-2) for putrescine and 0.073 A M-1 cm-2 for histamine, and detection limits (calculated as three times the signal-to-noise ratio) of 0.17 mu M for putrescine and 0.33 mu M for histamine. The optimized redox hydrogel-based biosensors were evaluated in terms of stability and selectivity, and were used for the determination of total amine content in fish samples kept for 10 days in different conditions.
引用
收藏
页码:1591 / 1597
页数:7
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