BRUCE: a program for the detection of transfer-messenger RNA genes in nucleotide sequences

被引：24

作者：

Laslett, D

Canback, B

Andersson, S

机构：

[1] Univ Uppsala, Dept Mol Evolut, SE-75236 Uppsala, Sweden

[2] Murdoch Univ, Perth, WA, Australia

来源：

NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 15期

关键词：

D O I：

10.1093/nar/gkf459

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A computer program, BRUCE, was developed for the identification of transfer-messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNA(Ala)-like secondary structure surrounding a short sequence encoding the tag peptide. In the 57 completely sequenced bacterial genomes where tmRNA genes have been reported previously, BRUCE identified all with no false positives. In addition, BRUCE found 99 of the 100 tmRNAs identified previously in other bacteria, red chloroplasts and cyanelles. The output of the program reports the proposed tRNA secondary structure, the tmRNA gene sequence and the tag peptide.

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收藏

页码：3449 / 3453

页数：5

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