Improved multiplex PCR method for the rapid detection of β-lactamase genes in Escherichia coli of animal origin

被引：17
作者：
Pomba, Constanca
Mendonca, Nuno
Costa, Marta
Louro, Deolinda
Baptista, Bruno
Ferreira, Monica
Correia, Jose Duarte
Canica, Manuela
机构：
[1] Univ Tecn Lisboa, Fac Vet Med, CIISA, P-1300477 Lisbon, Portugal
[2] Natl Inst Hlth Dr Ricardo Jorge, Ctr Bacteriol, Antibiot Resistance Unit, P-1649016 Lisbon, Portugal
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2006年 / 56卷 / 01期
关键词：
PCR; beta-lactamase genes; Escherichia coli; EXTENDED-SPECTRUM; SICK ANIMALS; RESISTANCE; SPAIN; DOGS;
D O I：
10.1016/j.diagmicrobio.2006.03.005
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%). (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：103 / 106
页数：4
相关论文
共 15 条
[11]   Heterogeneity among virulence and antimicrobial resistance gene profiles of extraintestinal Escherichia coli isolates of animal and human origin [J].
Maynard, C ;
Bekal, S ;
Sanschagrin, F ;
Levesque, RC ;
Brousseau, R ;
Masson, L ;
Larivière, S ;
Harel, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (12) :5444-5452
[12]   A novel sequence framework (blaTEM-1G) encoding the parental TEM-1 beta-lactamase [J].
Pomba-Féria, C ;
Caniça, M .
FEMS MICROBIOLOGY LETTERS, 2003, 220 (02) :177-180
[13]   Isolation of an SHV-12 β-lactamase-producing Escherichia coli strain from a dog with recurrent urinary tract infections [J].
Teshager, T ;
Domínguez, L ;
Moreno, MA ;
Saénz, Y ;
Torres, C ;
Cardeñosa, S .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (12) :3483-3484
[14]   Multiplex PCR for detection of aminoglycoside resistance genes in enterococci [J].
Vakulenko, SB ;
Donabedian, SM ;
Voskresenskiy, AM ;
Zervos, MJ ;
Lerner, SA ;
Chow, JW .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (04) :1423-1426
[15]   Impact of DNA polymerases and their buffer systems on quantitative real-time PCR [J].
Wolffs, P ;
Grage, H ;
Hagberg, O ;
Rådström, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (01) :408-411
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