Improved multiplex PCR method for the rapid detection of β-lactamase genes in Escherichia coli of animal origin

被引：17
作者：
Pomba, Constanca
Mendonca, Nuno
Costa, Marta
Louro, Deolinda
Baptista, Bruno
Ferreira, Monica
Correia, Jose Duarte
Canica, Manuela
机构：
[1] Univ Tecn Lisboa, Fac Vet Med, CIISA, P-1300477 Lisbon, Portugal
[2] Natl Inst Hlth Dr Ricardo Jorge, Ctr Bacteriol, Antibiot Resistance Unit, P-1649016 Lisbon, Portugal
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2006年 / 56卷 / 01期
关键词：
PCR; beta-lactamase genes; Escherichia coli; EXTENDED-SPECTRUM; SICK ANIMALS; RESISTANCE; SPAIN; DOGS;
D O I：
10.1016/j.diagmicrobio.2006.03.005
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%). (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：103 / 106
页数：4
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