A novel digital method to assess air space loss associated with acute lung injury in experimental acute pancreatitis

被引：1

作者：

Mukherjee, R. ^{[1
]}

Wen, L. ^{[1
,2
]}

Zhang, X. ^{[1
,2
]}

Bhattacharya, P. ^{[1
]}

Huang, W. ^{[1
,2
]}

Sutton, R. ^{[1
]}

机构：

[1] Univ Liverpool, Inst Translat Med, Liverpool Pancreatitis Res Grp, Liverpool, Merseyside, England

[2] Sichuan Univ, West China Hosp, Sichuan Prov Pancreatitis Ctr, Dept Integrated Tradit Chinese & Western Med, Chengdu, Sichuan, Peoples R China

来源：

PANCREATOLOGY | 2018年 / 18卷 / 05期

关键词：

Acute lung injury; Acute pancreatitis; FEATURES; MODELS;

D O I：

10.1016/j.pan.2018.04.017

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Objectives: Respiratory dysfunction and/or failure from acute lung injury (ALI) are common in acute pancreatitis (AP), but assessment of ALI in experimental AP has lacked standardisation. Methods: A range of experimental AP models induced in C57BL/6 mice with corresponding controls (n = 6/group). Full double lung or right lung specimens were taken for histopathological assessment and slides analysed by a pre-set pipeline using Aperio Scanner (Leica), ImageJ software and CellProfiler software. Findings were compared to other routinely assessed parameters. Results: Overall histopathological changes were similar between both lungs. Mean lung field occupancy was significantly different between moderate and severe CER-AP (21.9% v 27.5%, p < 0.05) and corresponded with lung MPO and local injury severity parameters and was mirrored for all models tested. Conclusion: We have developed a novel, simple method for assessment of ALI to improve measurement of systemic organ injury in experimental AP and contribute to preclinical drug development. (C) 2018 IAP and EPC. Published by Elsevier B.V. All rights reserved.

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页码：513 / 515

页数：3

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