Protective effects of the extracts of Barringtonia racemosa shoots against oxidative damage in HepG2 cells

被引：21

作者：

Kong, Kin Weng ^{[1
]}

Mat-Junit, Sarni ^{[1
]}

Aminudin, Norhaniza ^{[2
]}

Hassan, Fouad Abdulrahman ^{[3
]}

Ismail, Amin ^{[3
]}

Aziz, Azlina Abdul ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur, Malaysia

[2] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur, Malaysia

[3] Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Nutr & Dietet, Serdang 43400, Selangor, Malaysia

来源：

PEERJ | 2016年 / 4卷

关键词：

Polyphenols; Barringtonia racemosa; HPLC-ESI-MS; Antioxidant enzymes; Lipid peroxidation; Oxidative stress; TERT-BUTYL HYDROPEROXIDE; HUMAN HEPATOMA HEPG2; ANTIOXIDANT ACTIVITIES; HYDROGEN-PEROXIDE; HUMAN PLASMA; GALLIC ACID; STRESS; POLYPHENOLS; QUERCETIN; BIOAVAILABILITY;

D O I：

10.7717/peerj.1628

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Barringtonia racemosa is a tropical plant with medkinal values. In this study, the ability of the water extracts of the leaf (BLE) and stem (BSE) from the shoots Ito protect HepG2 cells against oxidative damage was studied. Five major polyphenolic compounds consisting of gallic acid, ellagic acid, protocatechuic acid, quercetin and kaempferol were identified using HPLC-DAD and ESI-MS. Cell viability assay revealed that BLE and BSE were non-cytotoxic (cell viabilities >80%) at concentration less than 250 mu g/ml and 500 jig/nil, respectively. BLE andl BSE improved cellular antioxidant status measured by FRAP assay and protected HepG2 cells against H2O2-induced cytotoxicity. The extracts also inhibited lipid peroxidation in HepG2 cells as well as the production of reactive oxygen species. BLE and BSE could also suppress the activities of superoxide dismutase and catalase during oxidative stress. The shoots of B. racemosa can be an alternative bioactive ingredient in the prevention of oxidative damage.

引用

页数：20

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