Genomic confirmation of vancomycin-resistant Enterococcus transmission from deceased donor to liver transplant recipient

被引：12
作者：
Bashir, Ali [1 ]
Attie, Oliver [1 ]
Sullivan, Mitchell [1 ]
Sebra, Robert [1 ]
Singh, Kavindra V. [2 ]
Altman, Deena [3 ]
Pak, Theodore [1 ]
Dutta, Jayeeta [1 ]
Chacko, Kieran [1 ]
Webster, Elizabeth [1 ]
Lewis, Martha [1 ]
Hamula, Camille [4 ]
Delli Carpini, Kristin W. [5 ]
Murray, Barbara E. [2 ]
Kasarskis, Andrew [1 ]
van Bakel, Harm [1 ]
Huprikar, Shirish [3 ]
机构：
[1] Icahn Sch Med Mt Sinai, Inst & Dept Genet & Genom Sci, New York, NY 10029 USA
[2] Univ Texas Med Sch, Div Infect Dis, Dept Med, Houston, TX USA
[3] Icahn Sch Med Mt Sinai, Dept Med, Div Infect Dis, New York, NY 10029 USA
[4] Icahn Sch Med, Dept Pathol, New York, NY USA
[5] LiveOnNY, New York, NY USA
来源：
PLOS ONE | 2017年 / 12卷 / 03期
关键词：
MOLECULAR EPIDEMIOLOGY; ALIGNMENT; INFECTIONS; ASSEMBLIES; SEQUENCE;
D O I：
10.1371/journal.pone.0170449
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In a liver transplant recipient with vancomycin-resistant Enterococcus (VRE) surgical site and bloodstream infection, a combination of pulsed-field gel electrophoresis, multilocus sequence typing, and whole genome sequencing identified that donor and recipient VRE isolates were highly similar when compared to time-matched hospital isolates. Comparison of de novo assembled isolate genomes was highly suggestive of transplant transmission rather than hospital-acquired transmission and also identified subtle internal rearrangements between donor and recipient missed by other genomic approaches. Given the improved resolution, whole-genome assembly of pathogen genomes is likely to become an essential tool for investigation of potential organ transplant transmissions.
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页数：10
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