New exopolysaccharides produced by Aureobasidium pullulans grown on glucosamine

被引:7
作者
Cescutti, P
Pupulin, R
Delben, F
Abbate, M
Dentini, M
Sparapano, L
Rizzo, R
Crescenzi, V
机构
[1] Univ Trieste, Dipartimento Biochim Biofis & Chim Macromol, I-34127 Trieste, Italy
[2] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy
[3] Univ Bari, Dipartimento Biol & Patol Vegetale, I-70126 Bari, Italy
关键词
Aureobasidium pullulans; polysaccharides; structure; NMR spectroscopy; lectin affinity chromatography;
D O I
10.1016/S0008-6215(02)00125-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The polysaccharides produced by Aureobasidium pullulans, grown using glucosamine as the carbon source, were investigated by means of methylation analysis, affinity chromatography and NMR spectroscopy. The results indicated that, besides a small amount of pullulan, this micro-organism was capable of producing-in low yields-mixtures of at least two different complex polysaccharides containing mainly mannose and galactose. H-1 NMR spectra of two fractions obtained by lectin affinity chromatography indicated that one polymer was constituted exclusively of mannose residues while the other contained both galactofuranosyl and mannopyranosyl residues. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:1203 / 1209
页数:7
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