The Effect of Different Water Extracts from Platycodon grandiflorum on Selected Factors Associated with Pathogenesis of Chronic Bronchitis in Rats

被引：16

作者：

Buchwald, Waldemar ^{[1
]}

Szulc, Michal ^{[2
]}

Baraniak, Justyna ^{[3
]}

Derebecka, Natalia ^{[4
]}

Kania-Dobrowolska, Malgorzata ^{[3
]}

Piasecka, Anna ^{[5
,6
]}

Bogacz, Anna ^{[3
]}

Karasiewicz, Monika ^{[7
]}

Bartkowiak-Wieczorek, Joanna ^{[8
]}

Kujawski, Radoslaw ^{[2
]}

Gryszczynska, Agnieszka ^{[3
]}

Kachlicki, Piotr ^{[5
]}

Dreger, Mariola ^{[9
]}

Ozarowski, Marcin ^{[9
]}

Krajewska-Patan, Anna ^{[1
]}

Gorska-Paukszta, Malgorzata ^{[9
]}

Kaminska, Ewa ^{[2
]}

Mikolajczak, Przemyslaw L. ^{[2
,3
]}

机构：

[1] Inst Nat Fibres & Med Plants, Dept Bot Breeding & Agr Technol Med Plants, Kolejowa 2, PL-62064 Plewiska, Poland

[2] Poznan Univ Med Sci, Dept Pharmacol, Rokietnicka 5a, PL-60806 Poznan, Poland

[3] Inst Nat Fibres & Med Plants, Dept Pharmacol & Phytochem, Kolejowa 2, PL-62064 Plewiska, Poland

[4] Adam Mickiewicz Univ, Inst Mol Biol & Biotechnol, Lab High Throughput Technol, Fac Biol, Umultowska 89, PL-61614 Poznan, Poland

[5] Polish Acad Sci, Inst Plant Genet, Dept Pathogen Genet & Plant Resistance, Metabol Team, Strzeszynska 34, PL-60479 Poznan, Poland

[6] Polish Acad Sci, Inst Bioorgan Chem, Noskowskiego 12-14, PL-61704 Poznan, Poland

[7] Poznan Univ Med Sci, Dept Prevent Med, Lab Int Hlth, Swiecickiego 6, PL-60781 Poznan, Poland

[8] Poznan Univ Med Sci, Dept Physiol, Swiecickiego 6, PL-60781 Poznan, Poland

[9] Inst Nat Fibres & Med Plants, Dept Biotechnol, Wojska Polskiego 71b, PL-60630 Poznan, Poland

来源：

MOLECULES | 2020年 / 25卷 / 21期

关键词：

Platycodon grandiflorum; extracts; bronchitis; mRNA expression; cytokines; saponins; MUCIN GENE-EXPRESSION; LIQUID-CHROMATOGRAPHY; GROWTH-FACTOR; NITRIC-OXIDE; IN-VIVO; SAPONINS; ROOTS; SECRETION; MUCUS; IDENTIFICATION;

D O I：

10.3390/molecules25215020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to assess the activity of extracts from Platycodon grandiflorum A. DC (PG) in a model of chronic bronchitis in rats. The research was carried out on three water extracts: E1 - from roots of field cultivated PG; E2 - from biotransformed roots of PG; E3 - from callus of PG. The extracts differed in saponins and inulin levels-the highest was measured in E3 and the lowest in E1. Identification of secondary metabolites was performed using two complementary LC-MS systems. Chronic bronchitis was induced by sodium metabisulfite (a source of SO2). Animals were treated with extracts for three weeks (100 mg/kg, intragastrically) and endothelial growth factor (VEGF), transforming growth factors (TGF-beta 1, -beta 2, -beta 3), and mucin 5AC (MUC5AC) levels were determined in bronchoalveolar lavage fluid, whereas C reactive protein (CRP) level was measured in serum. Moreover, mRNA expression were assessed in bronchi and lungs. In SO2-exposed rats, an elevation of the CRP, TGF-beta 1, TGF-beta 2, VEGF, and mucin was found, but the extracts' administration mostly reversed this phenomenon, leading to control values. The results showed a strong anti-inflammatory effect of the extracts from PG.

引用

页数：22

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