Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant:: characterization of the VanD5 operon

被引：33
作者：
Boyd, DA
Kibsey, P
Roscoe, D
Mulvey, MR
机构：
[1] Hlth Canada, Natl Microbiol Lab, Winnipeg, MB R3E 3R2, Canada
[2] Victoria Gen Hosp, Victoria, BC, Canada
[3] Vancouver Gen Hosp, Vancouver, BC, Canada
来源：
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2004年 / 54卷 / 03期
关键词：
glycopeptide resistance; van PCR; E; faecium;
D O I：
10.1093/jac/dkh391
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Objectives: To genotypically characterize the vancomycin resistance mechanism of Enterococcus faecium N03-0072, which was negative by PCR for the currently known van genotypes. Methods: PCR was used to amplify the entire vancomycin resistance operon and the complete nucleotide sequence was determined by dideoxy cycle sequencing. Results: Analysis revealed a VanD-type operon with 94% nucleotide identity to the VanD4 operon and 90% nucleotide identity to the VanD1/D3 operons. A set of universal primers was designed in order to identify all current vanD variants by PCR. Conclusions: E. faecium N03-0072 carries a new VanD-type operon, designated VanD5.
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收藏
页码：680 / 683
页数：4
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