Wheat peptide protects against ethanol-induced gastric mucosal damage through downregulation of TLR4 and MAPK

被引:28
作者
Yang, Xian [1 ]
Yang, Ligang [1 ]
Pan, Da [1 ]
Liu, Hechun [1 ]
Xia, Hui [1 ]
Wang, Shaokang [1 ]
Sun, Guiju [1 ]
机构
[1] Southeast Univ, Key Lab Environm Med & Engn, Dept Nutr & Food Hyg, Minist Educ,Sch Publ Hlth, Nanjing 210009, Peoples R China
关键词
Gastric mucosal damage; Ethanol; Wheat peptides; Anti-inflammatory; KAPPA-B ACTIVATION; INHIBITORY PEPTIDES; NITRIC-OXIDE; RECEPTOR; 4; ANTIOXIDANT; ULCER; EXPRESSION; INJURY; CELLS; RATS;
D O I
10.1016/j.jff.2020.104271
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Excessive alcohol consumption can induce gastric mucosal damage. The present work aimed to examine the protective effect of wheat peptides against ethanol-induced gastric mucosal damage in rats. Wheat peptides (WP) were administered daily by gavage for 30 days before the rats were treated with anhydrous ethanol (1 mL). Histological observations were performed, as well as the anti-oxidation effect and anti-inflammatory action. WP increased the superoxide dismutase activity and prostaglandin E-2 (PGE2) levels and decreased the malondialdehyde and interleukin-8 (IL-8) levels. WP suppressed gastric mucosa inflammation through the reduction of TNF-alpha, MPO, IL-6, and IL-1 beta. WP repressed the expression of caspase-3, and enhanced the expression of antiapoptotic protein EGFR. Furthermore, WP down-regulated protein expression levels of TLR4, MAPK and ERK, suggesting that WP have a potential effect to inhibit ethanol-induced inflammatory responses via blocking TLR4/MAPK/ERK signal transduction. The potential mechanism may be associated with enhancement of antioxidation, anti-inflammatory action and anti-apoptosis function.
引用
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页数:11
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