Closed-channel culture system for efficient and reproducible differentiation of human pluripotent stem cells into islet cells

被引：25

作者：

Hirano, Kunio ^{[1
]}

Konagaya, Shuhei ^{[2
]}

Turner, Alexander ^{[1
]}

Noda, Yuichiro ^{[1
]}

Kitamura, Shigeru ^{[1
]}

Kotera, Hidetoshi ^{[3
]}

Iwata, Hiroo ^{[2
]}

机构：

[1] ARKRAY Inc, Res & Dev Div, Kyoto 6020008, Japan

[2] Kyoto Univ, Inst Frontier Med Sci, Sakyo Ku, 53 Kawahara Cho, Kyoto 6068507, Japan

[3] Kyoto Univ, Fac Engn, Sakyo Ku, Yoshida Honmachi, Kyoto 6068501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 487卷 / 02期

关键词：

iPS cells; Induced differentiation; Microfluidics; Pancreatic islet cells; Microwells; Cellular aggregates; SUSPENSION-CULTURE; EMBRYOID BODIES; IN-VITRO; SIZE;

D O I：

10.1016/j.bbrc.2017.04.062

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality. (C) 2017 The Authors. Published by Elsevier Inc.

引用

页码：344 / 350

页数：7

共 21 条

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