Effects of Material Property and Heat Treatment on Nanomechanical Properties of Chitosan Films

被引:9
作者
Majd, Shervin [1 ]
Yuan, Youling [2 ]
Mishra, Sanjay [3 ]
Haggard, Warren O. [1 ]
Bumgardner, Joel D. [1 ]
机构
[1] Univ Memphis, Herff Coll Engn, Dept Biomed Engn, Memphis, TN 38152 USA
[2] Univ Louisville, JB Speed Sch Engn, Dept Bioengn, Louisville, KY 40292 USA
[3] Univ Memphis, Dept Phys, Memphis, TN 38152 USA
关键词
chitosan; chitin; nanomechanical properties; degree of deacetylation; molecular weight; ELASTIC-MODULUS; MECHANICAL-PROPERTIES; PHYSICAL-PROPERTIES; N-DEACETYLATION; CHITIN; MEMBRANES; TITANIUM; BEHAVIOR; HARDNESS;
D O I
10.1002/jbm.b.31284
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The influence of degree of deacetylation (DDA), molecular weight (MW), and heat treatment on nanomechanical properties of three different chitosan coatings was assessed. Chitosans (2.5 wt % in 2% acetic acid) were solution cast and bonded to glass slides, subjected to 40, 70, or 90 degrees C heat treatments for I h, and then allowed to air dry. Non-heat treated films were used as controls. The nanomechanical and structural properties were evaluated using quasi-static nanoindentation, X-ray diffraction (XRD), and atomic force microscopy (AFM). Nanoindentation results showed that the coating with the lowest MW and highest DDA (95.6% DDA and MW = 2.43 x 10(6) Da) had higher reduced modulus of elasticity, (E = 4.02 +/- 0.85 GPa) as compared to the other chitosans; 76.1% DDA/MW = 3.20 x 10(6) Da, E = 3.66 +/- 0.68 GPa, and 92.3% DDA/MW = 7.52 x 10(6) Da, E = 3.56 +/- 0.25 GPa; (n = 75, p < 0.05). Crystallinity index, estimated via XRD, ranged from 36.4% +/- 1% to 49.7% +/- 6.5%. AFM revealed the presence of different crystalline morphologies such as needle-like crystals, sheet-like crystals, and small spherulites. The heat treatments applied during solution casting of the chitosan coatings did not affect morphology or nanomechanical properties. In conclusion, nanomechanical properties of chitosan coatings varied with DDA and MW and may be important to cell/tissue interactions. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 283-289, 2009
引用
收藏
页码:283 / 289
页数:7
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