THE EFFECT OF ZATARIA MULTIFLORA BOISS. ESSENTIAL OIL ON GENE EXPRESSION OF ENTEROTOXIN C IN STAPHYLOCOCCUS AUREUS ATCC 6538

被引:7
作者
Parsaeimehr, Mahnoosh [1 ]
Basti, Afshin Akhondzadeh [2 ]
Misaghi, Ali [2 ]
Gandomi, Hassan [2 ]
Jebellijavan, Ashkan [1 ]
机构
[1] Semnan Univ, Fac Vet Med, Dept Food Hyg, Semnan 3513119111, Iran
[2] Univ Tehran, Fac Vet Med, Dept Food Hyg, Tehran, Iran
关键词
CHEMICAL-COMPOSITION; SUBINHIBITORY CONCENTRATIONS; SALMONELLA-TYPHIMURIUM; ANTIMICROBIAL ACTIVITY; THYMUS-VULGARIS; VIRULENCE; ANTIBACTERIAL; ANTIOXIDANT; GROWTH; ANTIVIRULENCE;
D O I
10.1111/jfpp.12401
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
This study was designed to evaluate the chemical constituents of Zataria multiflora Boiss. essential oil (EO) and its inhibitory effect on enterotoxin type C production by Staphylococcus aureus ATCC 6538. Moreover, the effect of this EO on gene expression of staphylococcal enterotoxin type C (SEC) was assessed. The effect of different concentrations of EO on enterotoxin C production was evaluated using 3-enzyme-linked immunosorbent assay. The reducing effect of EO subinhibitory concentrations on gene expression of enterotoxin was assayed using real-time polymerase chain reaction as well. EO at the level of 0.005% had no inhibitory effect on enterotoxin C production, and by increasing the subinhibitory concentration of the EO to 0.015%, an inhibitory effect was observed. Comparing the relative gene expression of SEC in control and treatment groups showed that the EO concentrations of 0.0075 and 0.015% had a significant downregulation (10.7 and 23.8 times, respectively) of sec gene.
引用
收藏
页码:1702 / 1709
页数:8
相关论文
共 48 条
  • [21] Development and evaluation of a rapid strategy to determine enterotoxin gene content in Staphylococcus aureus
    Fischer, Adrien
    Francois, Patrice
    Holtfreter, Silva
    Broeker, Barbara
    Schrenzel, Jacques
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2009, 77 (02) : 184 - 190
  • [22] Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species
    Gilles, Martin
    Zhao, Jian
    An, Min
    Agboola, Samson
    [J]. FOOD CHEMISTRY, 2010, 119 (02) : 731 - 737
  • [23] Gould G.W., 1995, FOOD PRESERVATION MO, P397
  • [24] Innovative Application of Mass Spectrometry for the Characterization of Staphylococcal Enterotoxins Involved in Food Poisoning Outbreaks
    Hennekinne, Jacques-Antoine
    Brun, Virginie
    De Buyser, Marie-Laure
    Dupuis, Alain
    Ostyn, Annick
    Dragacci, Sylviane
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (03) : 882 - 884
  • [25] Subinhibitory clindamycin differentially inhibits transcription of exoprotein genes in Staphylococcus aureus
    Herbert, S
    Barry, P
    Novick, RP
    [J]. INFECTION AND IMMUNITY, 2001, 69 (05) : 2996 - 3003
  • [26] Antibacterial and antifungal properties of essential oils
    Kalemba, D
    Kunicka, A
    [J]. CURRENT MEDICINAL CHEMISTRY, 2003, 10 (10) : 813 - 829
  • [27] Subinhibitory quinupristin/dalfopristin attenuates virulence of Staphylococcus aureus
    Koszczol, Carmen
    Bernardo, Katussevani
    Kroenke, Martin
    Krut, Oleg
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 58 (03) : 564 - 574
  • [28] Subinhibitory concentrations of β-lactam induce haemolytic activity in Staphylococcus aureus through the SaeRS two-component system
    Kuroda, Hiroko
    Kuroda, Makoto
    Cui, Longzhu
    Hiramatsu, Keiichi
    [J]. FEMS MICROBIOLOGY LETTERS, 2007, 268 (01) : 98 - 105
  • [29] Antistaphylococcal activity of Zataria multiflora essential oil and its synergy with vancomycin
    Mahboubi, M.
    Bidgoli, F. Ghazian
    [J]. PHYTOMEDICINE, 2010, 17 (07) : 548 - 550
  • [30] A new method for determining the minimum inhibitory concentration of essential oils
    Mann, CM
    Markham, JL
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 1998, 84 (04) : 538 - 544