In vitro and in vivo hypoglycemic effects of brown algal fucoidans

被引：123

作者：

Shan, Xindi ^{[1
,2
]}

Liu, Xin ^{[1
,2
]}

Hao, Jiejie ^{[1
,2
]}

Cai, Chao ^{[1
,2
]}

Fan, Fei ^{[1
,2
]}

Dun, Yunlou ^{[1
,2
]}

Zhao, Xiaoliang ^{[1
,2
]}

Liu, Xiaoxiao ^{[1
,2
]}

Li, Chunxia ^{[1
,2
]}

Yu, Guangli ^{[1
,2
]}

机构：

[1] Ocean Univ China, Minist Educ, Sch Med & Pharm, Key Lab Marine Drugs, Qingdao 266003, Peoples R China

[2] Ocean Univ China, Shandong Prov Key Lab Glycosci & Glycotechnol, Sch Med & Pharm, Qingdao 266003, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 82卷

关键词：

Fucoidan; alpha-Glucosidase; In vitro and in vivo studies; TYPE-2; DIABETES-MELLITUS; ALPHA-GLUCOSIDASE; FUCUS-VESICULOSUS; DB/DB MICE; AMYLASE; ACARBOSE; ANTICOAGULANT; HYPERGLYCEMIA; INHIBITOR; TOXICITY;

D O I：

10.1016/j.ijbiomac.2015.11.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inhibition of alpha-glucosidase is an effective therapeutic approach for type 2 diabetes mellitus that involves decreasing postprandial hyperglycemia. In the present study, the a-glucosidase and a-amylase inhibitory effects of 11 fucoidans extracted from different brown seaweeds were evaluated. Although no significant alpha-amylase inhibition was observed, fucoidan from Fucus vesiculosus (FvF) showed the highest alpha-glucosidase inhibitory activity, with an IC50 value of 67.9 mu g/mL. In addition, FvF at a concentration of 200 mu g/mL displayed very mild cytotoxicity to IEC-6 cells as indicated by the MTT assay. An in vivo study indicated that FvF decreased the fasting blood glucose and hemoglobin A1c (HbA1c) levels of db/db mice, with minimal effect on their weight. Therefore, our present in vitro and in vivo studies demonstrated that FvF could be a promising a-glucosidase inhibitor for the treatment of type 2 diabetes mellitus. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：249 / 255

页数：7

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