Molecular and functional characterization of the magnesium transporter gene ZmMGT12 in maize

被引：19

作者：

Li, Hongyou ^{[1
,2
]}

Liu, Chan ^{[1
]}

Zhou, Lina ^{[1
]}

Zhao, Zhuo ^{[1
]}

Li, Yihong ^{[1
]}

Qu, Min ^{[1
]}

Huang, Kaifeng ^{[1
]}

Zhang, Lu ^{[1
]}

Lu, Yanli ^{[1
]}

Cao, Moju ^{[1
]}

Gao, Shibin ^{[1
]}

Zhang, Suzhi ^{[1
]}

机构：

[1] Sichuan Agr Univ, Maize Res Inst, Minist Agr, Agr Dept,Key Lab Biol & Genet Improvement Maize S, Chengdu 611130, Sichuan, Peoples R China

[2] Guizhou Normal Univ, Res Ctr Buckwheat Ind Technol, Guiyang 550001, Guizhou, Peoples R China

来源：

GENE | 2018年 / 665卷

基金：

中国国家自然科学基金;

关键词：

Maize; Magnesium transporter; CorA; ZmMGT12; Arabidopsis; Overexpression; SALMONELLA-TYPHIMURIUM; POLLEN DEVELOPMENT; ESCHERICHIA-COLI; PROTEIN; FAMILY; CORA; HOMEOSTASIS; EXPRESSION; OVEREXPRESSION; RESISTANCE;

D O I：

10.1016/j.gene.2018.04.068

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Magnesium (Mg) is an essential mineral element for normal plant growth and development, and the CorA/MRS2/MGT-type Mg transporters play a significant role in maintaining Mg homeostasis in plants. In total, 12 maize CorA-like Mg2+ transporters have been identified, but none of them had been functionally characterized. Accordingly, we cloned and functionally characterized ZtnMGT12 from the maize CorA-like gene family. ZmMGT12 exhibited the structure typical of Mg2+ transporters, i.e., two conserved TM domains and a GMN tripeptide motif. ZmMGT12, Arabidopsis AtMGT6, and rice OsMRS2-6 shared high protein sequence identity and thus clustered in the same phylogenetic branch, suggesting that they could be homologs. A functional complementation assay in the Salmonella typhimurium MM281 mutant indicated that ZmMGT12 possessed Mg2+ transport ability. ZmMGT12 was expressed in roots, stems, and leaves, with the highest expression in leaves. Moreover, ZmMGT12 expression was induced by light and exhibited a circadian expression pattern. In addition, the expression level of ZmMGT12 in leaf tissue was related to chlorophyll synthesis. Overexpression of ZmMGT12 in Arabidopsis caused no phenotypic change in transgenic plants, including in fresh shoot weight, chlorophyll content, shoot Mg2+ content, and chloroplast Mg2+ content. Together, these results suggest that ZmMGT12 is a Mg2+ transporter and may play a role in Mg transport into chloroplasts.

引用

页码：167 / 173

页数：7

共 39 条

[1] The bacterial magnesium transporter CorA can functionally substitute for its putative homologue Mrs2p in the yeast inner mitochondrial membrane [J].