Biocatalytic application of nitrilases from Fusarium solani O1 and Aspergillus niger K10

被引：16

作者：

Winkler, Margit ^{[2
]}

Kaplan, Ondrej ^{[1
]}

Vejvoda, Vojtech ^{[1
]}

Klempier, Norbert ^{[2
]}

Martinkova, Ludmila ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Microbiol, Ctr Biocatalysis & Biotransformat, CZ-14220 Prague 4, Czech Republic

[2] Graz Univ Technol, Inst Organ Chem, A-8010 Graz, Austria

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2009年 / 59卷 / 04期

关键词：

Amino nitriles; Nitrilase; Substrate specificity; Enantioselectivity; Diastereoselectivity; RHODOCOCCUS-RHODOCHROUS NCIMB-11216; ASYMMETRIC HYDROLYSIS; MILD HYDROLYSIS; PURIFICATION; ENZYME; BIOTRANSFORMATION; ACIDS;

D O I：

10.1016/j.molcatb.2008.06.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nitrilases from Fusarium solani O1 and Aspergillus niger K10 showed a broad substrate specificity for carbocyclic and nonaromatic heterocyclic amino nitriles, the preferred substrates being five-membered gamma-amino nitrile (+/-)-1a, six-membered gamma-amino nitriles (+/-)-3a, (+/-)-5a and (+/-)-6a, pyrrolidine-3-carbonitriles (+/-)-9a and (+/-)-10a as well as piperidine-4-carbonitriles 14a and 15a. Both enzymes showed a strong diastereopreference for cis- vs. trans-gamma-amino nitriles. The electronic and steric effects of N-protecting groups affected the reactivity of the nitriles. Amides as by-products of the nitrilase-catalyzed reaction were produced from heterocyclic amino nitriles (+/-)-9a, (+/-)-10a, 14a and 15a by the A. niger enzyme but only from nitrile (+/-)-9a by the F. solani enzyme. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：243 / 247

页数：5

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