Anti-inflammatory action of high molecular weight Mytilus edulis hydrolysates fraction in LPS-induced RAW264.7 macrophage via NF-κB and MAPK pathways

被引：156
作者：
Kim, Young-Sang [1 ]
Ahn, Chang-Bum [2 ]
Je, Jae-Young [3 ]
机构：
[1] Pukyong Natl Univ, Dept Chem, Busan 48513, South Korea
[2] Chonnam Natl Univ, Div Food & Nutr, Gwangju 61186, South Korea
[3] Pukyong Natl Univ, Dept Marine Bio Convergence Sci, Busan 48547, South Korea
来源：
FOOD CHEMISTRY | 2016年 / 202卷
基金：
新加坡国家研究基金会;
关键词：
Mytilus edulis; Anti-inflammatory effect; Protein hydrolysate; NF-kappa B; MAPKs; RAW264.7; macrophages; PROTEIN HYDROLYSATE; ANTIOXIDANT; EXPRESSION; INFLAMMATION; INHIBITION; SYSTEM; SEEDS; SKIN; P38; JNK;
D O I：
10.1016/j.foodchem.2016.01.114
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Anti-inflammatory Mytilus edulis hydrolysates (MEHs) were prepared by peptic hydrolysis and MEH was further fractionated into three fractions based on molecular weight, namely >5 kDa, 1-5 kDa, and <1 kDa. The >5 kDa peptide fraction exerted the highest nitric oxide (NO) inhibitory activity and inhibited prostaglandin E-2 (PGE(2)) secretion in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Pretreatment with the >5 kDa peptide fraction markedly inhibited LPS-stimulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein and gene expressions. Stimulation by LPS induced the production of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and -1 beta (IL-1 beta), whereas co-treatment with the >5 kDa peptide fraction suppressed proinflammatory cytokine production. The >5 kDa peptide fraction inhibited the translocation of NF-kappa B (nuclear factor-kappa B) through the prevention of I kappa B alpha (inhibitory factor kappa B alpha) phosphorylation and degradation and also inhibited the MAPK signaling pathway in LPS-stimulated RAW264.7 macrophages. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：9 / 14
页数：6
相关论文
共 24 条
[1]   Kolaviron, a natural flavonoid from the seeds of Garcinia kola, reduces LPS-induced inflammation in macrophages by combined inhibition of IL-6 secretion, and inflammatory transcription factors, ERK1/2, NF-κB, p38, Akt, p-c-JUN and JNK [J].
Abarikwu, Sunny O. .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2014, 1840 (07) :2373-2381
[2]   Antioxidant and anti-inflammatory peptide fraction from salmon byproduct protein hydrolysates by peptic hydrolysis [J].
Ahn, Chang-Bum ;
Je, Jae-Young ;
Cho, Young-Sook .
FOOD RESEARCH INTERNATIONAL, 2012, 49 (01) :92-98
[3]   MAP kinases in inflammatory bowel disease [J].
Coskun, Mehmet ;
Olsen, Jorgen ;
Seidelin, Jakob Benedict ;
Nielsen, Ole Haagen .
CLINICA CHIMICA ACTA, 2011, 412 (7-8) :513-520
[4]   Angiotensin I-converting enzyme inhibitory activity of low-molecular-weight peptides from Atlantic salmon (Salmo salar L.) skin [J].
Gu, Rui-Zeng ;
Li, Chen-Yue ;
Liu, Wen-Ying ;
Yi, Wei-Xue ;
Cai, Mu-Yi .
FOOD RESEARCH INTERNATIONAL, 2011, 44 (05) :1536-1540
[5]   LPS induction of gene expression in human monocytes [J].
Guha, M ;
Mackman, N .
CELLULAR SIGNALLING, 2001, 13 (02) :85-94
[6]  
Ham Y.-M., 2015, J FUNCTIONAL FOODS, V17, P434
[7]   Signaling to NF-κB [J].
Hayden, MS ;
Ghosh, S .
GENES & DEVELOPMENT, 2004, 18 (18) :2195-2224
[8]   Amino acid composition and in vitro antioxidant and cytoprotective activity of abalone viscera hydrolysate [J].
Je, Jae-Young ;
Park, Soo Yeon ;
Hwang, Joung-Youl ;
Ahn, Chang-Bum .
JOURNAL OF FUNCTIONAL FOODS, 2015, 16 :94-103
[9]   NF-κB at the crossroads of life and death [J].
Karin, M ;
Lin, A .
NATURE IMMUNOLOGY, 2002, 3 (03) :221-227
[10]   Mast cells play a key role in the development of late airway hyperresponsiveness through TNF-α in a murine model of asthma [J].
Kim, Young-Suk ;
Ko, Hyun-Mi ;
Kang, Nam-In ;
Song, Chang-Ho ;
Zhang, Xin ;
Chung, Woo-Chan ;
Kim, Jae-Hong ;
Choi, In-Hak ;
Park, Young-Min ;
Kim, Gi-Young ;
Im, Shun-Young ;
Lee, Hern-Ku .
EUROPEAN JOURNAL OF IMMUNOLOGY, 2007, 37 (04) :1107-1115
123