The preparation of liposomes using compressed carbon dioxide: strategies, important considerations and comparison with conventional techniques

被引:26
作者
Bridson, R. H. [1 ]
Santos, R. C. D.
Al-Duri, B.
McAllister, S. M.
Robertson, J.
Alpar, H. O.
机构
[1] Univ Birmingham, Dept Chem Engn, Birmingham B15 2TT, W Midlands, England
[2] GlaxoSmithKline, Harlow CM19 5AW, Essex, England
[3] London Sch Pharm, Ctr Drug Delivery Res, London WC1N 1AX, England
关键词
D O I
10.1211/jpp.58.6.0008
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Numerous strategies are currently available for preparing liposomes, although no single method is ideal in every respect. Two methods for producing liposomes using compressed carbon dioxide in either its liquid or supercritical state were therefore investigated as possible alternatives to the conventional techniques currently used. The first technique used modified compressed carbon dioxide as a solvent system. The way in which changes in pressure, temperature, apparatus geometry and solvent flow rate affected the size distributions of the formulations was examined. In general, liposomes in the nano-size range with an average diameter of 200 nm could be produced, although some micron-sized vesicles were also present. Liposomes were characterized according to their hydrophobic drug-loading capacity and encapsulated aqueous volumes. The latter were found to be higher than in conventional techniques such as high-pressure homogenization. The second method used compressed carbon dioxide as an antisolvent to promote uniform precipitation of phospholipids from concentrated ethanolic solutions. Finely divided solvent-free phospholipid powders of saturated lipids could be prepared that were subsequently hydrated to produce liposomes with mean volume diameters of around 5 mu m.
引用
收藏
页码:775 / 785
页数:11
相关论文
共 50 条
[11]   Vapor-liquid equilibria of carbon dioxide with linoleic acid, α-tocopherol and triolein at elevated pressures [J].
Chen, CC ;
Chang, CMJ ;
Yang, PW .
FLUID PHASE EQUILIBRIA, 2000, 175 (1-2) :107-115
[12]   A high-performance liquid chromatographic method for the analysis of lipids from lyophilized formulations [J].
Choudhari, KB ;
Jayanthi, S ;
Murty, RB ;
Matharu, RP .
JOURNAL OF CHROMATOGRAPHY A, 1996, 724 (1-2) :343-347
[13]   LARGE VOLUME LIPOSOMES BY AN ETHER VAPORIZATION METHOD [J].
DEAMER, D ;
BANGHAM, AD .
BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 443 (03) :629-634
[14]  
DEBENEDETTI PG, 1993, FLUID PHASE EQUILIBR, V82, P311, DOI 10.1016/0378-3812(93)87155-T
[15]   POLYMERIC MATERIALS FORMED BY PRECIPITATION WITH A COMPRESSED FLUID ANTISOLVENT [J].
DIXON, DJ ;
JOHNSTON, KP ;
BODMEIER, RA .
AICHE JOURNAL, 1993, 39 (01) :127-139
[16]   Supercritical fluids as solvents for chemical and materials processing [J].
Eckert, CA ;
Knutson, BL ;
Debenedetti, PG .
NATURE, 1996, 383 (6598) :313-318
[17]   Crystallization of pure anhydrous polymorphs of carbamazepine by solution enhanced dispersion with supercritical fluids (SEDS™) [J].
Edwards, AD ;
Shekunov, BY ;
Kordikowski, A ;
Forbes, RT ;
York, P .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2001, 90 (08) :1115-1124
[18]   Production of insulin-loaded poly(ethylene glycol)/poly(l-lactide) (PEG/PLA) nanoparticles by gas antisolvent techniques [J].
Elvassore, N ;
Bertucco, A ;
Caliceti, P .
JOURNAL OF PHARMACEUTICAL SCIENCES, 2001, 90 (10) :1628-1636
[19]   The design and development of DaunoXome(R) for solid tumor targeting in vivo [J].
Forssen, EA .
ADVANCED DRUG DELIVERY REVIEWS, 1997, 24 (2-3) :133-150
[20]   Preparation of liposomes encapsulating water-soluble compounds using supercritical carbon dioxide [J].
Frederiksen, L ;
Anton, K ;
vanHoogevest, P ;
Keller, HR ;
Leuenberger, H .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1997, 86 (08) :921-928