Sensitive Water Ligand Observed via Gradient Spectroscopy with 19F Detection for Analysis of Fluorinated Compounds Bound to Proteins

被引：7

作者：

Furihata, Kazuo ^{[1
]}

Tashiro, Mitsuru ^{[2
]}

机构：

[1] Univ Tokyo, Div Agr & Agr Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Meisei Univ, Coll Sci & Technol, Dept Chem, Hino, Tokyo 1918506, Japan

来源：

MAGNETOCHEMISTRY | 2019年 / 5卷 / 02期

关键词：

F-19; NMR; NMR-based screening; fluorinated compound; water ligand observed via gradient spectroscopy (WaterLOGSY); TRANSFER DIFFERENCE NMR; BINDING-AFFINITY; IDENTIFICATION; RANGE;

D O I：

10.3390/magnetochemistry5020029

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The water ligand observed via a gradient spectroscopy type experiment with F-19 detection was applied to selectively detect fluorinated compounds with affinity to the target proteins. The F-19 signals of bound and unbound compounds were observed as opposite phases, which was advantageous to distinguish the binding state. The proposed NMR method was optimized based on the F-19{H-1} saturation transfer difference pulse sequence, and various inversion pulses for the water resonance were evaluated with the aim of high sensitivity.

引用

页数：6

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