Monitoring autophagy in live cells with a fluorescent light-up probe for G-quadruplex structures

被引：33

作者：

Chen, Hongbo ^{[1
,2
]}

Sun, Hongxia ^{[1
]}

Zhang, Suge ^{[1
,2
]}

Yan, Wenpeng ^{[3
]}

Li, Qian ^{[1
]}

Guan, Aijiao ^{[1
]}

Xiang, Junfeng ^{[4
]}

Liu, Meirong ^{[4
]}

Tang, Yalin ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, BNLMS, State Key Lab Struct Chem Unstable & Stable Speci, Inst Chem, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100080, Peoples R China

[4] Chinese Acad Sci, Inst Chem, Ctr Physiochem Anal & Measurement, Beijing 100190, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 35期

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

DNA; VISUALIZATION; STABILIZATION; RECOGNITION; DEGRADATION; LYSOSOMES; INHIBITOR; COMPLEX; SWITCH;

D O I：

10.1039/c9cc01263j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Monitoring autophagy can provide valuable insights into understanding human pathological mechanisms, developing novel drugs, and exploring autophagy control approaches. Here, we proposed a new strategy to specifically monitor autophagy by lighting up the G-quadruplex structures entering autolysosomes. Based on this strategy, we designed a small-molecule fluorescent probe for autophagy imaging. This work not only opens up a new way for developing autophagy probes, but also provides an effective tool for autophagy research.

引用

页码：5060 / 5063

页数：4

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