Use of microsatellite DNA markers to investigate the level of genetic diversity and population genetic structure of coconut (Cocos nucifera L.)

被引：69

作者：

Perera, L ^{[1
]}

Russell, JR ^{[1
]}

Provan, J ^{[1
]}

Powell, W ^{[1
]}

机构：

[1] Scottish Crop Res Inst, Dept Cell & Mol Genet, Dundee DD2 5DA, Scotland

来源：

GENOME | 2000年 / 43卷 / 01期

关键词：

coconut; Cocos nucifera; microsatellites; SSR; genetic diversity;

D O I：

10.1139/gen-43-1-15

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have used eight pairs of simple sequence repeat (SSR) primers to analyse the genetic diversity in 130 individuals of coconut (Cocos nucifera L.) comprising 75 tall individuals and 55 dwarf individuals, representing 94 different coconut ecotypes throughout the world. A total of 51 alleles were detected, with an average of 6.4 alleles per locus. Fifty alleles were detected in tall coconuts (talls; mean alleles/locus 6.3) compared with only 26 (mean/ locus 3.3) in dwarfs, and the average diversity value in talls (0.589) was also significantly higher than that in dwarfs (0.348). Using the eight SSRs we were able to uniquely discriminate 116 of the 130 individuals. A phenetic tree based on D-AD (absolute distance) values clustered individuals into five groups, each mainly composed of either talls or dwarfs. These results provide evidence in support of previous hypotheses concerning the dissemination of coconut, as well as important new information for conservation and breeding purposes.

引用

页码：15 / 21

页数：7

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