Direct immobilization of antibodies on Zn-doped Fe3O4 nanoclusters for detection of pathogenic bacteria

被引：27
作者：
Pal, Monalisa [1 ]
Lee, Sanghee [1 ]
Kwon, Donghoon [1 ]
Hwang, Jeongin [1 ]
Lee, Hyeonjeong [1 ]
Hwang, Seokyung [1 ]
Jeon, Sangmin [1 ]
机构：
[1] Pohang Univ Sci & Technol POSTECH, Dept Chem Engn, Pohang, Gyeongbuk, South Korea
来源：
ANALYTICA CHIMICA ACTA | 2017年 / 952卷
基金：
新加坡国家研究基金会;
关键词：
Zn-doped magnetic nanoclusters; Direct immobilization of antibodies; Sensitive and selective pathogenic bacteria detection; FLUORESCENT APTASENSOR; MAGNETIC NANOPARTICLES; FERRITE NANOPARTICLES; RAPID DETECTION; MRI CONTRAST; FACILE; IMMUNOASSAY; SEPARATION; FRAGMENTS; DELIVERY;
D O I：
10.1016/j.aca.2016.11.041
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Zinc-doped magnetic nanoclusters (Zn-MNCs) were synthesized and used to detect pathogenic bacteria in milk. Hydrothermally synthesized Zn-MNCs exhibited stronger magnetic properties than pure MNCs, which facilitated the magnetic separation from the sample using a permanent magnet. The presence of accessible Zn sites allows the direct immobilization of half-fragmented antibodies over Zn-MNCs through strong Zn-S bonds and prevents the tedious multiple steps of molecular functionalization or coating with costly noble metals prior to conjugation with an antibody. After the capture and magnetic separation of Salmonella in milk using the antibody-functionalized Zn-MNCs, the concentration of bacteria was determined with a portable ATP luminometer and the detection limit was found to be 10 CFU/mL. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：81 / 87
页数：7
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