DNA-barcoding identification of Dothistroma septosporum on Pinus contorta var. latifolia, P. banksiana and their hybrid in northern Alberta, Canada

被引：5

作者：

Feau, Nicolas ^{[1
]}

Ramsfield, Tod D. ^{[2
]}

Myrholm, Colin L. ^{[2
]}

Tomm, Bradley ^{[2
]}

Cerezke, Herb F. ^{[2
]}

Benowicz, Andy ^{[3
]}

Samis, Erica ^{[3
]}

Romano, Ashley ^{[3
]}

Dale, Angela L. ^{[1
,4
]}

Capron, Arnaud ^{[1
]}

Heinzelmann, Renate ^{[1
]}

Hamelin, Richard C. ^{[1
,5
]}

机构：

[1] Univ British Columbia, Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada

[2] Canadian Forest Serv, Northern Forestry Ctr, Nat Resources Canada, 5320-122 St, Edmonton, AB T6H 3S5, Canada

[3] Alberta Agr & Forestry, 9920-108 St, Edmonton, AB T5K 2M4, Canada

[4] GC New Construct Mat, FPInnovat, Vancouver, BC V6T 1Z4, Canada

[5] Univ Laval, Fac Foresterie & Geomat, Inst Biol Integrat & Syst IBIS, Quebec City, PQ G1V 0A6, Canada

来源：

CANADIAN JOURNAL OF PLANT PATHOLOGY | 2021年 / 43卷 / 03期

关键词：

DNA barcoding; Dothistroma needle blight; fungal identification; Koch’ s postulates; tree resistance; NEEDLE BLIGHT; RANGE;

D O I：

10.1080/07060661.2020.1829065

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The ascomycete fungi Dothistroma septosporum (Dorogin) M. Morelet and D. pini Hulbary are both responsible for Dothistroma needle blight disease (DNB) on pines. Using a DNA-barcoding approach, we confirmed that the DNB infection of lodgepole pine (Pinus contorta Dougl. var. latifolia Engelm.), jack pine (P. banksiana Lamb.) and their hybrid in four research sites in northern Alberta (Canada) was caused by D. septosporum, confirming that the range of D. septosporum extends east of the Rocky Mountains into Alberta. Analysis of mating-type (MAT) genes showed the presence of both mating types in each of the pine plantations, indicating that the pathogen has the potential to complete its lifecycle in this region. Controlled inoculation confirmed that the two native pines found in this area (P. contorta var. latifolia and P. banksiana) and their hybrid are susceptible to D. septosporum, suggesting that continuous monitoring of the disease needs to be carried out to prevent any future outbreak in natural stands.

引用

页码：472 / 479

页数：8

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