RNA-directed DNA methylation requires an AGO4-interacting member of the SPT5 elongation factor family

被引:117
作者
Bies-Etheve, Natacha [1 ]
Pontier, Dominique [1 ]
Lahmy, Sylvie [1 ]
Picart, Claire [1 ]
Vega, Danielle [1 ]
Cooke, Richard [1 ]
Lagrange, Thierry [1 ]
机构
[1] Univ Perpignan, Lab Genome & Dev Plantes, CNRS, Inst Rech Dev, F-66860 Perpignan, France
关键词
Arabidopsis; AGO4; SPT5; RdDM; silencing; POLYMERASE-IV; TRANSCRIPTION ELONGATION; ARABIDOPSIS-THALIANA; HETEROCHROMATIN; PROTEINS; SUBUNITS; COMPLEX; PLANTS; ROLES; SIRNA;
D O I
10.1038/embor.2009.31
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent studies have identified a conserved WG/GW-containing motif, known as the Argonaute (AGO) hook, which is involved in the recruitment of AGOs to distinct components of the eukaryotic RNA silencing pathways. By using this motif as a model to detect new components in plant RNA silencing pathways, we identified SPT5-like, a plant-specific AGO4-interacting member of the nuclear SPT5 (Suppressor of Ty insertion 5) RNA polymerase (RNAP) elongation factor family that is characterized by the presence of a carboxyterminal extension with more than 40 WG/GW motifs. Knockout SPT5-like mutants show a decrease in the accumulation of several 24-nt RNAs and hypomethylation at different loci revealing an implication in RNA-directed DNA methylation (RdDM). Here, we propose that SPT5-like emerged in plants as a facultative RNAP elongation factor. Its plant-specific origin and role in RdDM might reflect functional interactions with plant-specific RNA Pols required for RdDM.
引用
收藏
页码:649 / 654
页数:6
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