Distinct phosphorylation and dephosphorylation dynamics of protein arginine kinases revealed by fluorescent activity probes

被引：8

作者：

Jung, Hoyoung ^{[1
]}

Choi, Yigun ^{[1
]}

Lee, Donghee ^{[1
]}

Seo, Jeong Kon ^{[2
]}

Kee, Jung-Min ^{[1
]}

机构：

[1] UNIST, Dept Chem, Ulsan 44919, South Korea

[2] UNIST, Cent Res Facil UCRF, Ulsan 44919, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 52期

基金：

新加坡国家研究基金会;

关键词：

REAL-TIME; PHOSPHOARGININE; ASSAY;

D O I：

10.1039/c9cc03285a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein arginine (Arg) phosphorylation regulates stress responses and virulence in bacteria. With fluorescent activity probes, we show that McsB, a protein Arg kinase, can dephosphorylate phosphoarginine (pArg) residues to produce ATP from ADP, implicating the dynamic control of protein pArg levels by the kinase even without a phosphatase.

引用

页码：7482 / 7485

页数：4

共 50 条

[1] PHOSPHORYLATION AND DEPHOSPHORYLATION OF CYCLIC AMP-DEPENDENT PROTEIN KINASES
ERLICHMAN, J
ALFANO, J
ROSEN, OM
FEDERATION PROCEEDINGS, 1975, 34 (03) : 514 - 514
[2] Phosphorylation mechanism and structure of serine-arginine protein kinases
Ghosh, Gourisankar
Adams, Joseph A.
FEBS JOURNAL, 2011, 278 (04) : 587 - 597
[3] Progress of fluorescent probes for protein phosphorylation and glycosylation in atherosclerosis
Li, Jin
Xu, Jiheng
Zhao, Ruize
Zhang, Wei
Li, Ping
Zhang, Wen
Wang, Hui
Tang, Bo
CHEMISTRY-A EUROPEAN JOURNAL, 2024, 30 (18)
[4] Fluorescent probes of protein kinase activity
Shults, MD
Imperiali, B
BIOPOLYMERS, 2005, 80 (04) : 515 - 515
[5] Fluorescent probes of protein structure and dynamics.
Gray, HB
Dunn, AR
Winkler, JR
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2003, 226 : U230 - U230
[6] PHOSPHORYLATION AND DEPHOSPHORYLATION OF DISTINCT ISOFORMS OF THE HEAVY NEUROFILAMENT PROTEIN NF-H
SOUSSAN, L
CHERTOFF, R
MICHAELSON, DM
JOURNAL OF NEUROCHEMISTRY, 1994, 63 : S81 - S81
[7] PHOSPHORYLATION AND DEPHOSPHORYLATION OF DISTINCT ISOFORMS OF THE HEAVY NEUROFILAMENT PROTEIN NF-H
CHERTOFF, R
SOUSSAN, L
RODER, H
MICHAELSON, DM
CELLULAR AND MOLECULAR NEUROBIOLOGY, 1995, 15 (02) : 269 - 281
[8] The Secret Lives of Fluorescent Membrane Probes as Revealed by Molecular Dynamics Simulations
Filipe, Hugo A. L.
Moreno, Maria Joao
Loura, Luis M. S.
MOLECULES, 2020, 25 (15):
[9] Design and synthesis of fluorescent activity probes for protein phosphatases
Casey, Garrett R.
Beck, Jon R.
Stains, Cliff I.
CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT B, 2019, 622 : 29 - 53
[10] Structural modes of stabilization of permissive phosphorylation sites in protein kinases: Distinct strategies in Ser/Thr and Tyr kinases
Krupa, A
Preethl, G
Srinivasan, N
JOURNAL OF MOLECULAR BIOLOGY, 2004, 339 (05) : 1025 - 1039

← 1 2 3 4 5 →