Isolation of viable Neospora caninum from brains of wild gray wolves (Canis lupus)

被引：15

作者：

Dubey, J. P. ^{[1
]}

Jenkins, M. C. ^{[1
]}

Ferreira, L. R. ^{[1
]}

Choudhary, S. ^{[1
]}

Verma, S. K. ^{[1
]}

Kwok, O. C. H. ^{[1
]}

Fetterer, R. ^{[1
]}

Butler, E. ^{[2
]}

Carstensen, M. ^{[2
]}

机构：

[1] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA

[2] Minnesota Dept Nat Resources, Div Wildlife, Forest Lake, MN 55025 USA

来源：

VETERINARY PARASITOLOGY | 2014年 / 201卷 / 1-2期

关键词：

Neospora caninum; Gray wolf; Canis lupus; Bioassay; PCR; DEER ODOCOILEUS-VIRGINIANUS; TOXOPLASMA-GONDII; DEFINITIVE HOSTS; DIAGNOSIS; PCR; HYBRIDIZATION; TRANSMISSION; INFECTION; ANIMALS;

D O I：

10.1016/j.vetpar.2013.12.032

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Neospora caninum is a common cause of abortion in cattle worldwide. Canids, including the dog and the dingo (Canis familiaris), the coyote (Canis latrans), and the gray wolf (Canis lupus) are its definitive hosts that can excrete environmentally resistant oocysts in the environment, but also can act as intermediate hosts, harboring tissue stages of the parasite. In an attempt to isolate viable N. caninum from tissues of naturally infected wolves, brain and heart tissue from 109 wolves from Minnesota were bioassayed in mice. Viable N. caninum (NcWolfMn1, NcWolfMn2) was isolated from the brains of two wolves by bioassays in interferon gamma gene knockout mice. DNA obtained from culture-derived N. caninum tachyzoites of the two isolates were analyzed by N. caninum-specific Nc5 polymerase chain reaction and confirmed diagnosis. This is the first report of isolation of N. caninum from tissues of any wild canid host. Published by Elsevier B.V.

引用

页码：150 / 153

页数：4

共 22 条

[1]

Basso W, 2001, J PARASITOL, V87, P612, DOI 10.1645/0022-3395(2001)087[0612:FIONCF]2.0.CO

[2]

[3]

Butler Erika, 2011, Minnesota Department of Natural Resources Summaries of Wildlife Research Findings, P82

[4] Epidemiology and control of neosporosis and Neospora caninum [J].

Dubey, J. P. ;

Schares, G. ;

Ortega-Mora, L. M. .

CLINICAL MICROBIOLOGY REVIEWS, 2007, 20 (02) :323-+

[5] Isolation and RFLP genotyping of Toxoplasma gondii from the gray wolf (Canis lupus) [J].

Dubey, J. P. ;

Choudhary, S. ;

Ferreira, L. R. ;

Kwok, O. C. H. ;

Butler, E. ;

Carstensen, M. ;

Yu, L. ;

Su, C. .

VETERINARY PARASITOLOGY, 2013, 197 (3-4) :685-690

[6] Congenital transmission of Neospora caninum in white-tailed deer (Odocoileus virginianus) [J].

Dubey, J. P. ;

Jenkins, M. C. ;

Kwok, O. C. H. ;

Ferreira, L. R. ;

Choudhary, S. ;

Verma, S. K. ;

Villena, I. ;

Butler, E. ;

Carstensen, M. .

VETERINARY PARASITOLOGY, 2013, 196 (3-4) :519-522

[7] Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum [J].

Dubey, J. P. ;

Jenkins, M. C. ;

Rajendran, C. ;

Miska, K. ;

Ferreira, L. R. ;

Martins, J. ;

Kwok, O. C. H. ;

Choudhary, S. .

VETERINARY PARASITOLOGY, 2011, 181 (2-4) :382-387

[8] Neosporosis in animals-The last five years [J].

Dubey, J. P. ;

Schares, G. .

VETERINARY PARASITOLOGY, 2011, 180 (1-2) :90-108

[9] Seroprevalence of Neospora caninum and Toxoplasma gondii antibodies in white-tailed deer (Odocoileus virginianus) from Iowa and Minnesota using four serologic tests [J].

Dubey, J. P. ;

Jenkins, M. C. ;

Kwok, O. C. H. ;

Zink, R. L. ;

Michalski, M. L. ;

Ulrich, V. ;

Gill, J. ;

Carstensen, M. ;

Thulliez, P. .

VETERINARY PARASITOLOGY, 2009, 161 (3-4) :330-334

[10]

Dubey J.P., 2010, TOXOPLASMOSIS ANIMAL, P1

← 1 2 3 →