Nanoslit Confined DNA at Low Ionic Strengths

被引：18

作者：

Lee, Jinyong ^{[1
,2
]}

Kim, Sulhwa ^{[1
,2
]}

Jeong, Huisu ^{[3
]}

Jung, Gun Young ^{[3
]}

Chang, Rakwoo ^{[4
]}

Chen, Yeng-Long ^{[5
,6
,7
]}

Jo, Kyubong ^{[1
,2
]}

机构：

[1] Sogang Univ, Dept Chem, Seoul 121742, South Korea

[2] Sogang Univ, Interdisciplinary Program Integrated Biotechnol, Seoul, South Korea

[3] Gwanju Inst Sci & Technol, Sch Mat Sci & Engn, Kwangju, South Korea

[4] Kwangwoon Univ, Dept Chem, Seoul, South Korea

[5] Acad Sinica, Inst Phys, Taipei, Taiwan

[6] Natl Taiwan Univ, Dept Phys, Taipei, Taiwan

[7] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu, Taiwan

来源：

ACS MACRO LETTERS | 2014年 / 3卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

ELECTROSTATIC PERSISTENCE LENGTH; DYNAMICS; CONFORMATION; STATISTICS;

D O I：

10.1021/mz500396t

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

We present nanoslit confined DNA conformations at very low ionic strengths and a theory to explain most measurements for single DNA molecule size under strong nanoslit confinement. Very low ionic strength conditions not only increase the DNA persistence length dramatically, but also cause DNA molecules to swell to the extent that the effective diameter of DNA becomes larger than the nanoslit height. By accounting for these effects, our results and theory provide a reasonable clue for a current controversy regarding the dependence of the DNA conformation on slit height (h), persistence length (p), and effective diameter (w).

引用

页码：926 / 930

页数：5

共 32 条

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