Postweaning multisystemic wasting syndrome (PMWS) in the Philippines:: Porcine circovirus type 2 (PCV2) detection and characterization

被引:6
|
作者
Maldonado, J [1 ]
Ségales, J
Calsamiglia, M
Llopart, D
Sibila, M
Lapuz, Z
Riera, P
Artigas, C
机构
[1] Labs Hipra SA, Girona, Spain
[2] Univ Autonoma Barcelona, Fac Vet, Dept Sanitat Anat Anim, E-08193 Barcelona, Spain
[3] Philippine Coll Swine Practitioners, Manila, Philippines
来源
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2004年 / 66卷 / 05期
关键词
PCV2; PMWS;
D O I
10.1292/jvms.66.533
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Four swine facilities located in Northern Luzon, in the Philippines, showed lesions and clinical signs similar to those described in post weaning multisystemic wasting syndrome (PMWS). Post-mortem and histopathological examination revealed the presence of typical lymphoid lesions caused by porcine circovirus type 2 (PCV2). An in situ hybridization (ISH) technique allowed the detection of PCV2 nucleic acid associated with the lesions in target tissues. PCV2 DNA from paraffin embedded tissues was extracted, purified and sequenced. The phylogenetic analysis of the sequences obtained confirmed their identity, and grouped them into a PCV2 subgroup, together with some Canadian, French and Dutch isolates. This report is the first description of the presence of PMWS in the Philippines.
引用
收藏
页码:533 / 537
页数:5
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